Whole plant cell wall characterization using solution-state 2D NMR

By Shawn Mansfield, Hoon Kim, F. Lu, J. Ralph. Nature Protocols, 2012

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Quantitative structural characterization of the lignins from the stem and pith of bamboo (Phyllostachys pubescens) (2013, methodology): “Lignin was isolated according to Zhang et al. (2010b) , with minor modifi cations (Figure 1 ).”
Recent Advances in Characterization of Lignin Polymer by Solution-State Nuclear Magnetic Resonance (NMR) Methodology (2013, methodology): “Contours in this region are used to measure S/G/H ratios, as well as relative p-hydroxybenzoate (PB, in poplar), p-coumarate (PCA in corn) and ferulate (FA in corn) levels (Reprinted from [10].”
Structural analysis for lignin characteristics in biomass straw (2013, methodology): “The quantification of lignin H/S/G ratio has been reported by (13)C NMR and HSQC NMR analysis [115,116].”