Voltage-driven DNA translocations through a nanopore.

By Amit Meller, L. Nivón, D. Branton. Physical Review Letters, 2001

745 citations recorded by the source corpus.

We measure current blockade and time distributions for single-stranded DNA polymers during voltage-driven translocations through a single alpha-hemolysin pore. We use these data to determine the velocity of the polymers in the pore. Our measurements imply that, while polymers longer than the pore are translocated at a constant speed, the velocity of shorter polymers increases with decreasing length. This velocity is nonlinear with the applied field. Based on this data, we estimate the effective diffusion coefficient and the energy penalty for extending a molecule into the pore.

Subfields: Biomedical Engineering, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/522e9f85517ccb5ffff3a6c44461f65b2e35ba3b

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Citations in the corpus

Solid-state nanopores. (2007, background): “1 ms, yielding a residence time of about 1 μs per base (ref”
Transport phenomena in nanofluidics (2008, background): “Bayley and Cremer, 2001; Meller et al., 2001; Vercoutere et al., 2001; Vercoutere and Akeson, 2002".”
Three decades of nanopore sequencing (2016, background): “Although there are three particularly good sensing spots within the 5-nm-long stem 14,15 , experiments with differing length oligomers showed that all of the ~12 nucleobases that occupy the 5-nm-long stem during strand translocation are sensed and reflected in the current level of a blockade 29 .”
Origin of the electrophoretic force on DNA in solid-state nanopores (2009, neutral): “Voltage-driven transport of macromolecules through biologica”
Simulation of the electric response of DNA translocation through a semiconductor nanopore–capacitor (2006, background): “Many experiments have been done in this context [1, 4–10] demonstrating how the length of the DNA chain is related to the time of the ionic current blockade.”
Colloquium: Physical approaches to DNA sequencing and detection (2007, background): “…et al., 1999 p(A),p(C),p(dC),p(U),p(A)(C) ∼ 100 − 200 discrimination Meller et al., 2000 p(dA),p(dC),p(dA)(dC),p(dAdC),others 100 td ∝ 1 T2 , discrimination Meller et al., 2001 p(dA) 4-100 Ib transition at L ∼ Lp,vDNA Mathé et al., 2005 p(A),hairpin ∼ 50 3′ ↔ 5′ Butler et al., 2006…”
Characteristics of solid-state nanometre pores fabricated using a transmission electron microscope (2007, methodology): “…under stationary or time-varying forces, to detect DNA hybridization kinetics and to study the interaction of DNA with bound proteins using nanopore force spectroscopy (Meller et al 2001, Nakane et al 2002, Meller 2003, Bates et al 2003, Wang et al 2004, Mathe et al 2006, Hornblower et al 2007).”