Trycycler: consensus long-read assemblies for bacterial genomes

By Ryan R. Wick, Louise M. Judd, L. Cerdeira, J. Hawkey, G. Méric, B. Vezina, K. Wyres, Kathryn E. Holt. Genome Biology, 2021

454 citations recorded by the source corpus.

While long-read sequencing allows for the complete assembly of bacterial genomes, long-read assemblies contain a variety of errors. Here, we present Trycycler, a tool which produces a consensus assembly from multiple input assemblies of the same genome. Benchmarking showed that Trycycler assemblies contained fewer errors than assemblies constructed with a single tool. Post-assembly polishing further reduced errors and Trycycler+polishing assemblies were the most accurate genomes in our study. As Trycycler requires manual intervention, its output is not deterministic. However, we demonstrated that multiple users converge on similar assemblies that are consistently more accurate than those produced by automated assembly tools.

View Paper: https://www.semanticscholar.org/paper/5207609b670fd0d0f6d0409a8d46597eb084e2c3

Citations in the corpus

• Polypolish: Short-read polishing of long-read bacterial genome assemblies (2021, unknown): “Sequencing was previously described in Wick et al. (2021) [19].”