An efficient recombination system for chromosome engineering in Escherichia coli.

By Daiguan Yu, H. Ellis, E-Chiang Lee, Nancy A. Jenkins, N. Copeland, D. Court. Proceedings of the National Academy of Sciences of the United States of America, 2000

1,788 citations recorded by the source corpus.

Subfields: Ecology, Genetics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/5056f0cf6073db198b60ea75563104f40dd5bc43

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Citations in the corpus

Mini-λ: a tractable system for chromosome and BAC engineering (2003, methodology): “The frequencies of the CmR recombinants observed for galK were very similar to those found for the same targets using the defective prophage in strain DY331 when it was induced for 5 min (Yu et al., 2000).”
A highly efficient recombineering-based method for generating conditional knockout mutations. (2003, methodology): “…fragments (i.e., those carrying loxP sites and selection markers) to be inserted into DNA cloned on plasmids, BACs, or PACs via homologous recombination (Zhang et al. 1998; Muyrers et al. 1999; Datsenko and Wanner 2000; Murphy et al. 2000; Yu et al. 2000; Lee et al. 2001; Swaminathan et al. 2001).”
Interaction network containing conserved and essential protein complexes in Escherichia coli (2005, background): “Escherichia coli does not readily recombine exogenous linear DNA fragments into its chromosome, but expression of the lambda general recombination system (λ-Red) markedly enhances integratio”
Simple and highly efficient BAC recombineering using galK selection (2005, background): “This lower frequency of recombinants, when compared with the Nras G12D substitution experiment, is likely explained by the shorter homology arms used in this latter experiment, as it has been shown that the efficiency of recombination increase four orders of magnitude when homology length is increas”
An improved recombineering approach by adding RecA to λ Red recombination (2006, background): “In contrast, recombinogenic hosts like DY380 ( 10 ,18) or YZ2000 (7) carry the phage proteins on the E. coli chromosome.”