Argonaute2 complexes carry a population of circulating microRNAs independent of vesicles in human plasma

By Jason D. Arroyo, J. Chevillet, E. Kroh, I. K. Ruf, C. Pritchard, D. Gibson, P. S. Mitchell, C. F. Bennett, E. Pogosova-Agadjanyan, D. Stirewalt and 2 more. Proceedings of the National Academy of Sciences of the United States of America, 2011

3,252 citations recorded by the source corpus.

Subfields: Cancer Research, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/500a4e2ed5db8c1c82a5842e5ad1026e7a36871c

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Citations in the corpus

Standardization of sample collection, isolation and analysis methods in extracellular vesicle research (2013, neutral): “Others have reported dilution by factors of 3- to 10-fold (13,108,219).”
MicroRNAs in stress signaling and human disease (2012, background): “Recently, the majority of miRNAs in plasma were reported to be associated with Ago2 ribonucleo-protein complexes in a nonvesicular fraction, suggesting that miRNAs are released within functional miRNA-induced silencing complexes (Arroyo et al., 2011).”
Hypoxic enhancement of exosome release by breast cancer cells (2012, result): “Although miR-16 and miR-210 were amplified successfully from the exosome fractions, let7A failed to amplify, in spite of previous reports of its presence in exosomes [43,44].”
Characterization of human plasma-derived exosomal RNAs by deep sequencing (2013, background): “Recently, Argonaute 2 was reported to bind and protect miRNAs from degradation in the circulation [52].”
Deep sequencing of RNA from immune cell-derived vesicles uncovers the selective incorporation of small non-coding RNA biotypes with potential regulatory functions (2012, background): “Although the majority of circulating miRNAs in human plasma and serum were found to co-fractionate with proteins such as Argonaute2 (Ago2) and nucleophosmin, which have been suggested to protect miRNAs from degradation in RNase-rich environments (22,23), it is currently unknown if vesicle-free RNAs ”
Circulating microRNAs in exosomes indicate hepatocyte injury and inflammation in alcoholic, drug-induced and inflammatory liver diseases (2012, background): “The association of miR-122 with protein fractions has been demonstrated in healthy human plasma samples.(17) However, a recent study suggests that a majority of miRNAs in the serum and saliva is concentrated in exosomes.”
Biogenesis, secretion, and intercellular interactions of exosomes and other extracellular vesicles. (2014, background): “Of note, following the discovery of RNAs in exosomes (and EVs), other forms of extracellular miRNA have been reported: miRNAs have now been described in blood circulation as complexes with Ago2 protein (Arroyo et al. 2011) or with high-density lipoproteins (Vickers et al. 2011).”