Time course analysis of gene regulation under cadmium stress in rice
By Ippei Ogawa, H. Nakanishi, S. Mori, N. Nishizawa. Plant and Soil, 2009
121 citations recorded by the source corpus.
To elucidate the regulation of gene expression in response to cadmium (Cd) stress in rice (Oryza sativa), transcriptional changes in roots and shoots were investigated using a 22 K microarray covering 21,495 genes. Rice plants were exposed to 10 μM CdCl2 for 3 h or 1 μM CdCl2 for 24, 48, and 72 h, and 8 days in hydroponic culture. In roots, 1,207 genes were up-regulated, whereas 519 genes were down-regulated by more than twofold under 10 μM Cd stress for 3 h. Compared with roots, the shoots had fewer Cd-responsive genes. The expression of genes such as those encoding cytochrome P450 family proteins, heat shock proteins, and glutathione S-transferase was strongly induced. Genes encoding proteins involved in signal transduction, including transcription factors such as DREB and NAC, and protein kinases, were also induced. Genes involved in photosynthesis were mainly down-regulated after 3 h of stress. Genes for the synthesis of nicotianamine and 2′-deoxymugineic acid were induced in roots under 1 μM Cd stress for 8 days, suggesting the occurrence of iron deficiency under longer-term Cd stress. Cd-regulated transporter genes included PDR and MATE family transporters, which were strongly up-regulated in roots, especially under 10 μM Cd stress, suggesting their role in Cd detoxification via export of Cd from the cytoplasm. Their modification may potentially lead to the development of low-Cd rice, which contributes to human health as well as high-Cd rice useful for phytoremediation.
Subfields: Plant Science
View Paper: https://www.semanticscholar.org/paper/4ee69d484e35ff83a8df5b74778269c8f517be3e
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In Plant Science
- M. Fujita
- M. A. Hossain
- N. Nishizawa
- Khurram Bashir
- Yasuhiro Ishimaru
- J. A. Teixeira da Silva
- H. Nakanishi
- P. Piyatida
Citations in the corpus
- Rice phenolics efflux transporter 2 (PEZ2) plays an important role in solubilizing apoplasmic iron (2011, background): “We obtained 50 independent T-DNA mutants from the rice T-DNA insertion sequence database (http:// www.postech.ac.kr/life/pfg/risd/index.html) potentially related to Cd transport on the basis of changes in expression in response to Cd (Ogawa et al. 2009; Jeon et al. 2000; Jeong et al. 2006).”
- Molecular Mechanism of Heavy Metal Toxicity and Tolerance in Plants: Central Role of Glutathione in Detoxification of Reactive Oxygen Species and Methylglyoxal and in Heavy Metal Chelation (2012, background): “[349] also found strong induction of the OsGST”