Resolution criteria for three dimensional reconstruction

By M. Heel, G. Harauz. 1986

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Macromolecular structure determination using X-rays, neutrons and electrons: recent developments in Phenix (2019, methodology): “The value is obtained by analyzing the Fourier shell correlation (FSC) for two cryo-EM half-maps binned in resolution shells (van Heel & Harauz, 1986).”
Single-particle electron cryo-microscopy: towards atomic resolution (2000, background): “(6) This formula, first derived in 1968 (in a slightly different form (DeRosier & Klug, 1968)) for single axis tilt experiments, has an equivalent for randomly oriented projections, which differs only in the multiplicative constant (van Heel & Harauz, 1986).”
Measuring the optimal exposure for single particle cryo-EM using a 2.6 Å reconstruction of rotavirus VP6 (2015, background): “Calculating the Fourier Shell Correlation (FSC) (van Heel and Harauz, 1986) between the VP6 map and the refined atomic model demonstrated a resolution of ∼2.7 Å (Figure 3A).”