Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics.

By Xianlin Han, R. Gross. Journal of Lipid Research, 2003

753 citations recorded by the source corpus.

Lipidomics is a rapidly expanding research field in which multiple techniques are utilized to quantitate the hundreds of chemically distinct lipids in cells and determine the molecular mechanisms through which they facilitate cellular function. Recent developments in electrospray ionization mass spectrometry (ESI/MS) have made possible, for the first time, the precise identification and quantification of alterations in a cell's lipidome after cellular perturbations. This review provides an overview of the essential role of ESI/MS in lipidomics, presents a broad strategy applicable for the generation of lipidomes directly from cellular extracts of biological samples by ESI/MS, and summarizes salient examples of strategies utilized to conquer the lipidome in physiologic signaling as well as pathophysiologically relevant disease states. Because of its unparalleled sensitivity, specificity, and efficiency, ESI/MS has provided a critical bridge to generate highly accurate data that fingerprint cellular lipidomes to facilitate insight into the functional role of subcellular membrane compartments and microdomains in mammalian cells. We believe that ESI/MS-facilitated lipidomics has now opened a critical door that will greatly increase our understanding of human disease.

Subfields: Molecular Biology, Spectroscopy

View Paper: https://www.semanticscholar.org/paper/4da1e23a8cddb7701c8cab510300e660fafbe8b9

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Citations in the corpus

Metabolomics: back to basics (2008, neutral): “For the non-polar compounds lipidomics has been developed as a sub-metabolomics approach (Han and Gross 2003).”
Mass spectrometry-based metabolomics. (2007, methodology): “Neutral lipids, such as triacylglycerols are not readily ionized by ESI, however they can be detected as ammonium, lithium, or sodium adducts with ESI in positive mode (Duffin, Henion, & Shieh, 1991; Hsu & Turk, 1999; Han & Gross, 2005).”
A novel informatics concept for high-throughput shotgun lipidomics based on the molecular fragmentation query language (2011, background): “…lipidomics methodology implies that total lipid extracts from cells or tissues are directly infused into a tandem mass spectrometer and the identification of individual species relies on their accurately determined masses and/or MS/MS spectra acquired from corresponding precursor ions [6-8].”