Enhanced isolation of SARS-CoV-2 by TMPRSS2-expressing cells
By S. Matsuyama, Naganori Nao, K. Shirato, Miyuki Kawase, Shinji Saito, I. Takayama, N. Nagata, T. Sekizuka, H. Katoh, Fumihiro Kato and 8 more. Proceedings of the National Academy of Sciences of the United States of America, 2020
1,379 citations recorded by the source corpus.
A novel betacoronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), which caused a large respiratory outbreak in Wuhan, China in December 2019, is currently spreading across many countries globally. Here, we show that a TMPRSS2-expressing VeroE6 cell line is highly susceptible to SARS-CoV-2 infection, making it useful for isolating and propagating SARS-CoV-2. Our results reveal that, in common with SARS- and Middle East respiratory syndrome-CoV, SARS-CoV-2 infection is enhanced by TMPRSS2.
Subfields: Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/4d4d1b842637a0e0b07838a5a5641a3e36e5de91
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In Infectious Diseases
Citations in the corpus
- Tracking Changes in SARS-CoV-2 Spike: Evidence that D614G Increases Infectivity of the COVID-19 Virus (2020, neutral): “TMPRSS2, a type-II transmembrane serine protease, cleaves the viral Spike after receptor binding to enhance entry of MERSCoV, SARS-CoV, and SARS-CoV-2 (Hoffmann et al., 2020b; Kleine-Weber et al., 2018; Matsuyama et al., 2020; Millet and Whittaker, 2014; Park et al., 2016; Shulla et al., 2011; Zang ”
- Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development (2020, methodology): “These findings are consistent with previous studies showing that VeroE6/TMPRSS2 and VeroE6 cells can efficiently support the replication of SARS-CoV-2 (4, 5).”
- TMPRSS2 and furin are both essential for proteolytic activation of SARS-CoV-2 in human airway cells (2020, background): “Recent studies indicate that TMPRSS2 is also involved in SARSCoV-2 S protein activation (20, 21).”