Syrian hamsters as a small animal model for SARS-CoV-2 infection and countermeasure development

By M. Imai, K. Iwatsuki-Horimoto, M. Hatta, S. Loeber, P. Halfmann, N. Nakajima, Tokiko Watanabe, M. Ujie, Kenta Takahashi, Mutsumi Ito and 24 more. Proceedings of the National Academy of Sciences of the United States of America, 2020

998 citations recorded by the source corpus.

Significance Since SARS-CoV-2 emerged in China, it has spread rapidly around the world. Effective vaccines and therapeutics for SARS-CoV-2−induced disease (coronavirus disease 2019;COVID-19) are urgently needed. We found that SARS-CoV-2 isolates replicate efficiently in the lungs of Syrian hamsters and cause severe pathological lesions in the lungs of these animals similar to commonly reported imaging features of COVID-19 patients with pneumonia. SARS-CoV-2−infected hamsters mounted neutralizing antibody responses and were protected against rechallenge with SARS-CoV-2. Moreover, passive transfer of convalescent serum to naïve hamsters inhibited virus replication in their lungs. Syrian hamsters are a useful small animal model for the evaluation of vaccines, immunotherapies, and antiviral drugs. At the end of 2019, a novel coronavirus (severe acute respiratory syndrome coronavirus 2; SARS-CoV-2) was detected in Wuhan, China, that spread rapidly around the world, with severe consequences for human health and the global economy. Here, we assessed the replicative ability and pathogenesis of SARS-CoV-2 isolates in Syrian hamsters. SARS-CoV-2 isolates replicated efficiently in the lungs of hamsters, causing severe pathological lung lesions following intranasal infection. In addition, microcomputed tomographic imaging revealed severe lung injury that shared characteristics with SARS-CoV-2−infected human lung, including severe, bilateral, peripherally distributed, multilobular ground glass opacity, and regions of lung consolidation. SARS-CoV-2−infected hamsters mounted neutralizing antibody responses and were protected against subsequent rechallenge with SARS-CoV-2. Moreover, passive transfer of convalescent serum to naïve hamsters efficiently suppressed the replication of the virus in the lungs even when the serum was administrated 2 d postinfection of the serum-treated hamsters. Collectively, these findings demonstrate that this Syrian hamster model will be useful for understanding SARS-CoV-2 pathogenesis and testing vaccines and antiviral drugs.

Subfields: Epidemiology, Infectious Diseases

View Paper: https://www.semanticscholar.org/paper/c8379e62ed257508f59ff84c10fe10acd2586ae3

Citations in the corpus

• SARS-CoV-2 D614G Variant Exhibits Efficient Replication ex vivo and Transmission in vivo (2020, neutral): “To evaluate the impact of the D614G substitution in SARS-CoV-2 respiratory transmissibility, we set up eight pairs of hamsters for each virus similar to previously studies (11, 12).”
• A mouse-adapted model of SARS-CoV-2 to test COVID-19 countermeasures (2020, neutral): “Several animal models for SARS-CoV-2 have been reported, with varying degrees of viral replication and clinical disease, including ACE2 transgenic mice 9–11 and virally transduced ACE2 mice 8,12,13 , which express human ACE2, ferrets 14 , hamsters 15,16 and non-human primates 17–19 .”