Biocompatibility of gold nanoparticles and their endocytotic fate inside the cellular compartment: a microscopic overview.

By R. Shukla, V. Bansal, Minakshi Chaudhary, A. Basu, R. Bhonde, M. Sastry. Langmuir, 2005

1,637 citations recorded by the source corpus.

Macrophages are one of the principal immune effector cells that play essential roles as secretory, phagocytic, and antigen-presenting cells in the immune system. In this study, we address the issue of cytotoxicity and immunogenic effects of gold nanoparticles on RAW264.7 macrophage cells. The cytotoxicity of gold nanoparticles has been correlated with a detailed study of their endocytotic uptake using various microscopy tools such as atomic force microscopy (AFM), confocal-laser-scanning microscopy (CFLSM), and transmission electron microscopy (TEM). Our findings suggest that Au(0) nanoparticles are not cytotoxic, reduce the production of reactive oxygen and nitrite species, and do not elicit secretion of proinflammatory cytokines TNF-alpha and IL1-beta, making them suitable candidates for nanomedicine. AFM measurements suggest that gold nanoparticles are internalized inside the cell via a mechanism involving pinocytosis, while CFLSM and TEM studies indicate their internalization in lysosomal bodies arranged in perinuclear fashion. Our studies thus underline the noncytotoxic, nonimmunogenic, and biocompatible properties of gold nanoparticles with the potential for application in nanoimmunology, nanomedicine, and nanobiotechnology.

Subfields: Electronic, Optical and Magnetic Materials, Materials Chemistry

View Paper: https://www.semanticscholar.org/paper/4c454b638ff3bdd53e4633e8f83ef957a0fa059d

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Citations in the corpus

Mesoporous silica nanoparticles deliver DNA and chemicals into plants. (2007, background): “1 a,c), which not only serve as a biocompatible capping agen”
Toxicity and cellular uptake of gold nanoparticles: what we have learned so far? (2010, positive): “Similar results were obtained using gold nanoparticles (spheres, 3.5 nm in diameter) on immune system cell lines ( Shukla et al. 2005 ).”
Surface-structure-regulated cell-membrane penetration by monolayer-protected nanoparticles. (2008, background): “In contrast, most nanoparticles are trapped in endosome”
Direct and indirect CeO2 nanoparticles toxicity for Escherichia coli and Synechocystis (2009, background): “Therefore, several papers have recently studied the impact of nanoparticles on animals or mammalian cells (Derfus et al. 2004; Kirchner et al. 2005; Shukla et al. 2005; Franklin et al. 2007; Yacobi et al. 2007)”
Gold nanoparticles as novel agents for cancer therapy. (2012, neutral): “fication and use in biomedical applications [12].”