Targeted Genome Editing Across Species Using ZFNs and TALENs

By A. Wood, Te-Wen Lo, B. Zeitler, C. Pickle, E. Ralston, Andrew H Lee, Rainier Amora, Jeffrey C. Miller, Elo Leung, Xiangdong Meng and 5 more. Science, 2011

660 citations recorded by the source corpus.

Engineered nucleases target specific DNA sequences for gene disruption in nonmodel organisms. Evolutionary studies necessary to dissect diverse biological processes have been limited by the lack of reverse genetic approaches in most organisms with sequenced genomes. We established a broadly applicable strategy using zinc finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) for targeted disruption of endogenous genes and cis-acting regulatory elements in diverged nematode species.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4b8eb5ee716fa80c0825e9d2b29d476a504f8002

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In Molecular Biology

Citations in the corpus

Double nicking by RNA-guided CRISPR Cas9 for enhanced genome editing specificity (2013, background): “…(TALENs) (Boch et al., 2009; Christian et al., 2010; Miller et al., 2011; Moscou and Bogdanove, 2009; Reyon et al., 2012; Sanjana et al., 2012; Wood et al., 2011; Zhang et al., 2011), wherein DNA cleavage requires synergistic interaction of two independent specificity-encoding DNAbinding…”
Optimization of scarless human stem cell genome editing (2013, methodology): “To eliminate repeats, we computationally evolved the nucleotides sequence of TALE RVD arrays to minimize the number of sequence repeats while maintaining the amino acid composition.”
FLASH Assembly of TALENs Enables High-Throughput Genome Editing (2012, positive): “6 This framework has been used to construct nucleases that function efficiently in nematodes, 18 zebrafish, 21, 22 rats, 20 and human somatic 6, 15, 17 and pluripotent stem cells, 19 and we therefore predict that FLASH-assembled TALENs will also likely show high activities and high success rates in…”
Discovery and resupply of pharmacologically active plant-derived natural products: A review (2015, methodology): “of these species as disease models has been additionally expanded by recent implementation of the techniques of gene editing in both nematodes (Wood et al., 2011) and zebrafish (Huang et al.”
RNA-guided genome editing in plants using a CRISPR-Cas system. (2013, background): “Among them, zinc-finger nucleases (ZFNs) and transcription activatorlike effector nucleases (TALENs) are the two most commonly used sequence-specific chimeric proteins (Pabo et al., 2001; Wood et al., 2011).”
A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants. (2015, background): “In recent years various genetic tools, especially those involving sequence-specific nucleases for creating targeted DSB, have emerged as the major breakthrough for site-specific genome editing (Li et al., 2011; Wood et al., 2011; Cong et al., 2013).”