High-resolution profiling of histone methylations in the human genome.

By A. Barski, Suresh Cuddapah, Kairong Cui, Tae-Young Roh, Dustin E. Schones, Zhibin Wang, Gang Wei, I. Chepelev, Keji Zhao. Cell, 2007

6,976 citations recorded by the source corpus.

Histone modifications are implicated in influencing gene expression. We have generated high-resolution maps for the genome-wide distribution of 20 histone lysine and arginine methylations as well as histone variant H2A.Z, RNA polymerase II, and the insulator binding protein CTCF across the human genome using the Solexa 1G sequencing technology. Typical patterns of histone methylations exhibited at promoters, insulators, enhancers, and transcribed regions are identified. The monomethylations of H3K27, H3K9, H4K20, H3K79, and H2BK5 are all linked to gene activation, whereas trimethylations of H3K27, H3K9, and H3K79 are linked to repression. H2A.Z associates with functional regulatory elements, and CTCF marks boundaries of histone methylation domains. Chromosome banding patterns are correlated with unique patterns of histone modifications. Chromosome breakpoints detected in T cell cancers frequently reside in chromatin regions associated with H3K4 methylations. Our data provide new insights into the function of histone methylation and chromatin organization in genome function.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/4ae155011e8d7ab3da901acbd449510da9a533b1

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Citations in the corpus

Chromatin-state discovery and genome annotation with ChromHMM (2017, methodology): “…applied the chromatin-state modeling framework to genome-wide ChIP-seq maps of 41 epigenomic modifications and related factors from a single-cell type, CD4T cells 5,7,9 , showing that their combinations and relative genomic positions were highly informative of distinct biological functions.”
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Nucleosome positioning and gene regulation: advances through genomics (2009, background): “This basic pattern has also held true for metazoan”
Charting histone modifications and the functional organization of mammalian genomes (2011, background): “An example is the comprehensive work by Keji Zhao's group to profile 39 different histone methylation and acetylation marks genome-wide in human CD4 + T cell”