DNA-free genome editing in plants with preassembled CRISPR-Cas9 ribonucleoproteins

By J. Woo, Jungeun Kim, S. Kwon, Claudia Corvalán, S. Cho, Hyeran Kim, Sang-Gyu Kim, Sang-Tae Kim, Sunghwa Choe, Jin-Soo Kim. Nature Biotechnology, 2015

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Genome Editing of Plants (2017, background): “Such approaches have been successful to create targeted mutations in plants (Luo et al., 2015; Woo et al., 2015).”
Efficient and transgene-free genome editing in wheat through transient expression of CRISPR/Cas9 DNA or RNA (2016, positive): “…complex in protoplasts can result in the production of speciﬁcally targeted, transgene-free mutants in the T0 generation in several plant species 6 , this method is unlikely efﬁcient in major cereal crops (such as wheat, maize, rice and sorghum) as the regeneration from protoplasts has been…”
Genome Editing—Principles and Applications for Functional Genomics Research and Crop Improvement (2017, background): “Recently, preassembled Cas9 protein-gRNA ribonucleoproteins have been directly delivered into cells to successfully edit genes in several crops, such as rice and wheat (Woo et al., 2015; Svitashev et al., 2016; Liang et al., 2017).”