Biocatalytic Asymmetric Synthesis of Chiral Amines from Ketones Applied to Sitagliptin Manufacture

By C. Savile, J. Janey, E. Mundorff, Jeffrey C. Moore, Sarena Tam, W. Jarvis, J. Colbeck, A. Krebber, F. J. Fleitz, J. Brands and 3 more. Science, 2010

1,484 citations recorded by the source corpus.

Subfields: Inorganic Chemistry, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/17bb936ece1a4c82745614f18a1c2e251951bdf7

How researchers see this paper

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In Inorganic Chemistry

1. A. Demain
2. J. Adrio

In Molecular Biology

1. Hak-Sung Kim
2. J. Choi
3. Sang-Soo Han
4. A. Demain
5. J. Adrio
6. Stefan Lutz
7. John M. Woodley
8. R. Sheldon

Citations in the corpus

• Beyond directed evolution - semi-rational protein engineering and design (2010, background): “Simultaneously targeting several structural regions within an enzyme, an impressive example of multi-parameter optimization was the recently reported redesign of ω -transaminase from Arthrobacter sp . into an industrial biocatalyst for the production of the antidiabetic compound sitagliptin [20••].”
• Artificial Metalloenzymes: Reaction Scope and Optimization Strategies. (2018, background): “6 have supplanted well-established large-scale processes based on homogeneous catalysts.(8) This technique relies on the efficient high-throughput screening of genetic diversity to identify and characterize functionally evolved mutants.”
• Engineering the third wave of biocatalysis (2012, methodology): “(R)-8-d1-1-(4,4,5,5,6,6,6-d6-5hydroxyhexyl)-3,7-dimethyl-1Hpurine-2,6-(3H,7H)dione (17) A recombinantly expressed enzyme was used to produce this deuterium containing pentoxyfylline analogue on the gram scale.”
• Microbial Enzymes: Tools for Biotechnological Processes (2014, methodology): “Several rounds of directed evolution were applied to create an engineered amine transaminase with a 40,000-fold increase in activity [160].”