Engineering the third wave of biocatalysis

By U. Bornscheuer, G. Huisman, R. Kazlauskas, Stefan Lutz, Jeffrey C. Moore, K. Robins. Nature, 2012

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Consensus protein design (2016, methodology): “Directed evolution and informatics-based rational design are transforming the field of protein engineering (Arnold and Volkov, 1999; Jiang et al., 2008; Lutz, 2010; Brustad and Arnold, 2011; Bornscheuer et al., 2012; Joh et al., 2014; Woolfson et al., 2015).”
Industrial applications of enzyme biocatalysis: Current status and future aspects. (2015, background): “Since the mid 1990s, in vitro version of Darwinian evolution, so called directed evolution, has made a great contribution to the development of enzymes with great potential (Bornscheuer et al., 2012).”
Artificial Metalloenzymes: Reaction Scope and Optimization Strategies. (2018, background): “Toward the latter point, for example, it is not obvious that phylogenetic tools used to generate targeted "smart libraries" of natural enzymes are applicable to ArMs given that the function of the two are completely unrelated.(163) Nonetheless, adapting the full arsenal of library methodologies that”