A highly efficient Escherichia coli-based chromosome engineering system adapted for recombinogenic targeting and subcloning of BAC DNA.

By E-Chiang Lee, Daiguan Yu, J. M. D. Velasco, L. Tessarollo, D. Swing, D. Court, Nancy A. Jenkins, N. Copeland. Genomics, 2001

1,272 citations recorded by the source corpus.

Subfields: Genetics, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/47b29485101bae0a59a9921463c3eafec73314a7

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Citations in the corpus

Mini-λ: a tractable system for chromosome and BAC engineering (2003, methodology): “coli, like DY331 and DY380, harboring a defective E prophage have been generated where the expression of phage genes exo, bet, and gam, under control of a temperature-sensitive CI-repressor (Yu et al., 2000; Lee et al., 2001), has been used to induce homologous recombination.”
Distinct roles for Hedgehog and canonical Wnt signaling in specification, differentiation and maintenance of osteoblast progenitors (2006, methodology): “…GFP::Cre construct from the plasmid pTGCK (M. T. Valerius and A.P.M., unpublished), a derivative of plasmids triTAUBi (Kistner et al., 1996), pBS592 (Le et al., 1999) and pIGCN21 (Lee et al., 2001), using high-fidelity platinum pfx polymerase, following the manufacturer’s directions (Invitrogen).”
Non-canonical inflammasome activation targets caspase-11 (2011, methodology): “Correctly targeted BAC DNA was transformed into Flp-expressing SW105 cell”
Transcriptional control of adipose lipid handling by IRF4 (2011, positive): “A C57Bl/6 mouse bacterial artificial chromosome (90G21) containing the Adipoq gene was transformed into the recombinogenic EL250 bacteria cells, and homologous recombination was performed as described elsewhere (Lee et al., 2001).”
The Chemokine Stromal Cell-Derived Factor-1 Regulates GABAergic Inputs to Neural Progenitors in the Postnatal Dentate Gyrus (2008, methodology): “To generate SDF-1-BAC reporter vector, monomeric red fluorescence protein 1 (mRFP1) was inserted at the end of the SDF-1 coding sequence by -Red-mediated recombination with slight modifications (Lee et al., 2001).”
Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development (2008, methodology): “, 1999) and FRT-Kan-FRT from pICGN21 (Lee et al., 2001).”