Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.

By A. Brand, Norbert Perrimon. 1993

Subfields: Computational Biology & Bioinformatics

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An Integrated Micro- and Macroarchitectural Analysis of the Drosophila Brain by Computer-Assisted Serial Section Electron Microscopy (2010, background): “, Gal4 lines; [23]), one can target specific cell types, labeling these cells, or manipulating them genetically.”
Drosophila strategies to study psychiatric disorders. (2013, background): “In addition to versatile genetics allowing temporal and spatial control of any gene’s expression [22], the ability to quickly generate genetic variants with altered gene expression patterns is potentially useful in studying disorders such as schizophrenia and autism, because these diseases are likel”
GAL4 system in drosophila: A fly geneticist's swiss army knife (2002, background): “To facilitate the generation of transgenic responder and driver lines Brand and Perrimon (1993) constructed a number of vectors (Fig.”
Papilin in development; a pericellular protein with a homology to the ADAMTS metalloproteinases. (2000, methodology): “Full-size Drosophilapapilin cDNA was generated by PCR. NotI sites were introduced into the 5′ end and 3′end primers and the resulting fragment was cloned into the NotI site of a pUAST vector (Brand and Perrimon, 1993).”
Model Organisms are Not (Theoretical) Models (2015, background): “for targeted gene expression first developed in Drosophila (Brand and Perrimon [1993]) has been extended for use in other organisms, including the frog Xenopus laevis, Zebrafish (Danio rerio), and mice.”
The Fly as a Model for Neurodegenerative Diseases: Is It Worth the Jump? (2007, unknown): “This technique is efficient and transgenic animals are produced at a high rate. The ability to generate multiple transgenic lines for each construct statistically diminishes any potential integration site effects. As in higher animals, fusion genes have been made, with a recent trend in life imaging”
Mg2+ Block of Drosophila (2012, unknown): “Therefore, we used an elav-GAL4/UAS-GFP (elav/GFP) transgenic line (Brand and Perrimon, 1993), which expresses GFP in neurons, to characterize endogenous dNMDARs in pupal primary cultured neurons (Figure 1A).”
Fringe is a glycosyltransferase that modifies Notch (2000, methodology): “We examined the biological activity of fngDEE by using an ectopic expression assa”
Early development of the Drosophila brain: IV. Larval neuropile compartments defined by glial septa (2003, background): “Their structure and significance in maintaining neurons of the adult brain has been demonstrated recently by Buchanan and Benzer (1993).”