Gene transfer by lentiviral vectors is limited by nuclear translocation and rescued by HIV-1 pol sequences

By A. Follenzi, L. Ailles, S. Bakovic, M. Geuna, L. Naldini. Nature Genetics, 2000

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A Versatile Viral System for Expression and Depletion of Proteins in Mammalian Cells (2009, background): “It is provided by the packaging plasmid [49,50,51] and the Tat protein is produced and secreted into the media by the packaging cell line.”
Molecular evolution of a novel hyperactive Sleeping Beauty transposase enables robust stable gene transfer in vertebrates (2009, background): “In particular, we and others have shown that lentiviral transduction efficiencies can be increased by incorporating more robust promoters [26] or cis -acting elements [27-29] into the vector backbone or by altering the transduction conditions [30].”
Retroviral DNA Integration: ASLV, HIV, and MLV Show Distinct Target Site Preferences (2004, methodology): “To produce HIV vector particles, 293T cells were cotransfected with three plasmids: one encoding the HIV vector segment (p156RRLsinPPTCMVGFPWPRE) (Follenzi et al. 2000), the second, the packaging construct (pCMVdeltaR9) (Naldini et al. 1996), and the third, the gene for VSV-G (pMD.”