Fluorescence in situ hybridization (FISH) with rRNA-targeted oligonucleotide probes

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Microbial Methane Formation from Hard Coal and Timber in an Abandoned Coal Mine (2008, methodology): “Fluorescence in situ Hybridization (FISH) A specific detection of Archaea was carried out by FISH as described in detail by Amann et al. (1990) and Pernthaler et al. (2001) using the general probe Arch915 (5′- GTG CTC CCC CGC CAA TTC CT-3′).”
Prokaryotic community structure and heterotrophic production in a river-influenced coastal arctic ecosystem (2006, background): “To ensure a counting error of <10%, between 600 and 800 DAPI-stained objects were counted per sample (Pernthaler et al. 2001).”
Microbial Community Analysis of Opalinus Clay Drill Core Samples from the Mont Terri Underground Research Laboratory, Switzerland (2007, methodology): “Samples were filtered and CARD-FISH was applied on filters following previously described protocols ( Pernthaler et al. 2001 , 2002 ; Schippers et al.”
Sulfate reducing bacteria in microbial mats: Changing paradigms, new discoveries (2006, background): “This allows researchers to visualize population differences under an epifluorescent or confocal microscope (Pernthaler et al., 2001).”
Fluorescence In Situ Hybridization and Catalyzed Reporter Deposition for the Identification of Marine Bacteria (2002, unknown): “Millipore) as described previously (37).”