Development and Use of Microsatellite Markers for Germplasm Characterization in Quinoa (Chenopodium quinoa Willd.)

By S. L. Mason, M. R. Stevens, E. Jellen, A. Bonifacio, D. Fairbanks, C. Coleman, R. McCarty, A. Rasmussen, P. Maughan. 2005

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Morphological Analysis of Quinoa Germplasm Grown Under Lowland Desert Conditions (2011, background): “Risi and Galwey (1989a) reported low values of LTN for highland quinoas than for valley quinoas in Peru, with a mean value of 7.”
Re‐Introduction of Quínoa into Arid Chile: Cultivation of Two Lowland Races under Extremely Low Irrigation (2009, background): “Genetic characterizations separate the quinoa landraces into two broad ecotypes or categories: Altiplano and Lowland (Mason et al. 2005).”
Assessment of genetic diversity in the USDA and CIP-FAO international nursery collections of quinoa (Chenopodium quinoa Willd.) using microsatellite markers (2007, methodology): “…banding pattern (i.e. all markers amplified a single polymorphic band with the exception of one marker (AAT71) that amplified a second monomorphic band) in prescreen tests and exhibited high heterozygosity values (range 0.33–0.85; mean ¼ 0.59; Mason et al., 2005) were selected for genotyping.”
Assessment of genetic diversity patterns in Chilean quinoa (Chenopodium quinoa Willd.) germplasm using multiplex fluorescent microsatellite markers (2009, background): “Multiplex fluorescent SSR analysis Twenty di- or tri-nucleotide microsatellites reported by Mason et al. (2005), and Jarvis (unpublished data), were selected based on high heterozygosity values (Mason et al. 2005), reproducibility, and clear amplification of a single amplicon.”