Disruption of KCC2 reveals an essential role of K-Cl cotransport already in early synaptic inhibition.

By C. Hübner, V. Stein, I. Hermans-Borgmeyer, T. Meyer, K. Ballanyi, T. Jentsch. Neuron, 2001

631 citations recorded by the source corpus.

Synaptic inhibition by GABA(A) and glycine receptors, which are ligand-gated anion channels, depends on the electrochemical potential for chloride. Several potassium-chloride cotransporters can lower the intracellular chloride concentration [Cl(-)](i), including the neuronal isoform KCC2. We show that KCC2 knockout mice died immediately after birth due to severe motor deficits that also abolished respiration. Sciatic nerve recordings revealed abnormal spontaneous electrical activity and altered spinal cord responses to peripheral electrical stimuli. In the spinal cord of wild-type animals, the KCC2 protein was found at inhibitory synapses. Patch-clamp measurements of embryonic day 18.5 spinal cord motoneurons demonstrated an excitatory GABA and glycine action in the absence, but not in the presence, of KCC2, revealing a crucial role of KCC2 for synaptic inhibition.

Subfields: Cellular and Molecular Neuroscience, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/43e0bfb9de193824161dffeffb631d47565f7e4a

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Cellular and Molecular Neuroscience

Aleksandra Badura

In Molecular Biology

Citations in the corpus

Molecular physiology and pathophysiology of electroneutral cation-chloride cotransporters. (2005, background): “4) Impaired functional properties Bartter syndrome-BSC1 (384) Anderman syndrome-KCC3 (184) 5 1”
Raising cytosolic Cl− in cerebellar granule cells affects their excitability and vestibulo‐ocular learning (2012, positive): “The neuronal expression of Nkcc1 generally decreases postnatally (Hu¨bner et al , 2001a; Mikawa et al , 2002), but cerebellar GCs retain substantial Nkcc1 expression throughout adult-hood (Kanaka et al , 2001; Hu¨bner et al , 2001a; Mikawa et al , 2002).”