Arabinose and xylose fermentation by recombinant Saccharomyces cerevisiae expressing a fungal pentose utilization pathway
By M. Bettiga, Oskar Bengtsson, B. Hahn-Hägerdal, M. Gorwa-Grauslund. Microbial Cell Factories, 2009
646 citations recorded by the source corpus.
BackgroundSustainable and economically viable manufacturing of bioethanol from lignocellulose raw material is dependent on the availability of a robust ethanol producing microorganism, able to ferment all sugars present in the feedstock, including the pentose sugars L-arabinose and D-xylose. Saccharomyces cerevisiae is a robust ethanol producer, but needs to be engineered to achieve pentose sugar fermentation.ResultsA new recombinant S. cerevisiae strain expressing an improved fungal pathway for the utilization of L-arabinose and D-xylose was constructed and characterized. The new strain grew aerobically on L-arabinose and D-xylose as sole carbon sources. The activities of the enzymes constituting the pentose utilization pathway(s) and product formation during anaerobic mixed sugar fermentation were characterized.ConclusionPentose fermenting recombinant S. cerevisiae strains were obtained by the expression of a pentose utilization pathway of entirely fungal origin. During anaerobic fermentation the strain produced biomass and ethanol. L-arabitol yield was 0.48 g per gram of consumed pentose sugar, which is considerably less than previously reported for D-xylose reductase expressing strains co-fermenting L-arabinose and D-xylose, and the xylitol yield was 0.07 g per gram of consumed pentose sugar.
Subfields: Biomedical Engineering, Endocrinology, Diabetes and Metabolism, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/4327b2c1f7fa504872f0eb5e2c698b1a86321d37
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Citations in the corpus
- Enhanced acid tolerance in Lactobacillus casei by adaptive evolution and compared stress response during acid stress (2012, background): “The undissociated lactate entering the cytoplasm will dissociate and disrupt the intracellular pH (pHi) and anion pool of the cytoplasm, which affected the integrity of purine bases and resulted in the denaturing of essential enzymes inside the cell [7,8].”
- Hemicelluloses for fuel ethanol: A review. (2010, neutral): “The expression of LAD/LXR allows the conversion of L-arabitol into L-xylulose and then to xylitol, reducing L-arabitol accumulation (Bettiga et al., 2009).”
- Value-adding to cellulosic ethanol: lignin polymers. (2011, positive): “…Rattanachomsri et al., 2009), developing enzymes capable of converting the range of sugars produced by digestion of hemicelluloses to ethanol, (Bettiga et al., 2009; Yano et al., 2009) and improving the pre-treatment process for the fractionation of cellulose, hemicellulose and lignin from…”