Integrative Structure and Functional Anatomy of a Nuclear Pore Complex
By Seung Joong Kim, Javier Fernandez-Martinez, I. Nudelman, Yi Shi, Wenzhu Zhang, Barak Raveh, Thurston Herricks, Brian D. Slaughter, Joanna A. Hogan, Paula Upla and 22 more. Nature, 2018
455 citations recorded by the source corpus.
Nuclear pore complexes play central roles as gatekeepers of RNA and protein transport between the cytoplasm and nucleoplasm. However, their large size and dynamic nature have impeded a full structural and functional elucidation. Here we determined the structure of the entire 552-protein nuclear pore complex of the yeast Saccharomyces cerevisiae at sub-nanometre precision by satisfying a wide range of data relating to the molecular arrangement of its constituents. The nuclear pore complex incorporates sturdy diagonal columns and connector cables attached to these columns, imbuing the structure with strength and flexibility. These cables also tie together all other elements of the nuclear pore complex, including membrane-interacting regions, outer rings and RNA-processing platforms. Inwardly directed anchors create a high density of transport factor-docking Phe-Gly repeats in the central channel, organized into distinct functional units. This integrative structure enables us to rationalize the architecture, transport mechanism and evolutionary origins of the nuclear pore complex.
Subfields: Molecular Biology, Structural Biology
View Paper: https://www.semanticscholar.org/paper/4216b8a2f1007062a9c17df5ed67a81caaf40e8b
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Citations in the corpus
- Nuclear pores as versatile reference standards for quantitative superresolution microscopy (2019, unknown): “We first validated this approach by counting Nup82, which is present also in 16 copies 45,46 , and by counting Nup82 and Nup188 molecules together within a strain where both were tagged with mMaple (Fig.”
- AI-based structure prediction empowers integrative structural analysis of human nuclear pores (2022, result): “Within the IR, NUP188 and NUP205 localize to the outer and inner subcomplexes, respectively, consistent with previous analysis in other species (12, 22, 23).”