Cultivar Identification in Olive Based on RAPD Markers

By G. Besnard, Catherine Marie Breton, P. Baradat, B. Khadari, A. Bervillé. 2001

91 citations recorded by the source corpus.

One hundred and thirteen olive (Olea europaea L.) accessions were characterized using randomly amplified polymorphic DNA (RAPD) markers. Forty-five polymorphic RAPD markers were obtained enabling us to distinguish 102 different RAPD profiles. The approximate estimation of the probability of obtaining the same RAPD profile for two different trees was between 6.75 × 10 -5 and 4.82 × 10 -14 . A dendrogram was constructed using Ward's minimum variance algorithm based on chi-square distances. This led to a more clear-cut classification of profiles than the classical approach of unweighted pair group method with arithmetic average. Twenty-four clusters of RAPD profiles were shown in Ward's dendrogram. Reliability of the dendrogram structure was checked using variance analysis. RAPD data exhibited an acceptable resolving power for cultivar identification. A combination of three primers was proposed for rapid molecular identification of cultivars in collections and in nurseries. Olive (Olea europaea) is of great socioeconomic importance in the Mediterranean basin. Its cultivation is expanding because of increased demand for olive oil. A great number of olive cultivars (presumed clones) are grown throughout the world. Several hundred supposedly clonal accessions are described in the main countries of the Mediterranean basin. In France about 150 cultivars are registered (Andlauer, 1997). During the history of olive cultivation, as for other tree species, different cultivars may have been given the same name, whereas a cultivar may have been named differently in different countries. Therefore, cultivar and denomination (usual or local name) are ambiguous terms. Vegetative propagation of trees of agronomic interest has produced numerous clones (Zohary and Hopf, 1994). Conse- quently, genetic uniformity within a given cultivar, and thus, within a denomination, is expected. Cultivar identification based on phenological and morphological phenotypes from field or nursery observations may not be adequate to assign cultivar identity due to environmental effects on traits. Moreover, the

Subfields: Genetics, Molecular Biology, Organic Chemistry

View Paper: https://www.semanticscholar.org/paper/401265801cef8142ec22118d0b09101f820294aa

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Genetics

1. M. C. Almadanim
2. A. Martins
3. V. Gemas
4. R. Tenreiro
5. P. Fevereiro
6. K. Sefc

In Molecular Biology

1. Catherine Marie Breton
2. M. Tersac
3. A. Bervillé
4. K. Sefc

In Organic Chemistry

1. M. C. Almadanim
2. A. Martins
3. V. Gemas
4. R. Tenreiro
5. P. Fevereiro

Citations in the corpus

• Genetic evidence of intra-cultivar variability within Iberian olive cultivars (2004, background): “…grown all over the world, estimated as about 1200 with over 3000 synonyms (Rugini and Lavee, 1992) and the increasing demand for olive oil (Besnard et al., 2001a; http://www.internationaloliveoil. org/downloads/consomation1_ang.PDF) com- bined, increase the importance of the correct…”
• Genetic diversity and gene flow between the wild olive (oleaster, Olea europaea L.) and the olive: several Plio‐Pleistocene refuge zones in the Mediterranean basin suggested by simple sequence repeats analysis (2006, methodology): “…were selected according to their common name in different countries (Greece, Italy, Crete, Cyprus), and in each of the 23 groups of the cultivars (Besnard et al., 2001b), which represent most of the cultivated olive diversity, and consequently the oleaster diversity available and from which they…”
• Substantial genetic diversity in cultivated Moroccan olive despite a single major cultivar: a paradoxical situation evidenced by the use of SSR loci (2008, unknown): “In Spain, 17 main cultivars are grown depending on the region (Barranco 1995).”
• Genetic diversity in the Olive tree (Olea europaea L. subsp. europaea) cultivated in Portugal revealed by RAPD and ISSR markers (2004, methodology): “The geographic genetic structure was not ex- ‘Nabali’ ‘Souri’ and ‘Chelmal’ from Israel (Wiesman clusively observed among cultivars of different coun- et al. 1998; Besnard et al. 2001b); ‘Sabina’ and tries, but also within cultivars of the same country, yet ‘Ghermana’ from Corsica, France (Besnard e”