Mapping a wild tomato introgression associated with tomato yellow leaf curl virus resistance in a cultivated tomato line.

By P. Hanson, D. Bernacchi, S. Green, S. Tanksley, V. Muniyappa, A. Padmaja, Huei-Mei Chen, G. Kuo, D. Fang, Jen-tzu Chen. 2000

163 citations recorded by the source corpus.

Tomato yellow leaf curl virus (TYLCV), a heterogeneous complex of whitefly-vectored geminiviruses, is a serious production constraint of tomato (Lycopersicon esculentum Mill.) in Asia, the Middle East, and the Americas. In this study we report on mapping of a DNA fragment introgressed into cultivated tomato presumably from the wild species L. hirsutum Humb. and Bonpl. and found to be associated with TYLCV resistance. To locate introgressions of wild tomato alleles in TYLCV-resistant tomato line H24, its DNA was digested with six restriction enzymes and probed with 90 RFLP markers evenly spaced throughout the genome. This polymorphism survey revealed the presence of one wild tomato introgression each on chromosomes 8 and 11. Plants of a F 2 cross between H24 and a susceptible tomato line were probed with randomly amplified polymorphic DNA (RFLP) markers linked to the targeted regions and F 3 families were developed by self- pollination of F2 plants that carried none, one, or both introgressions in either homozygous or heterozygous states. Plants of F3 families, parents, and control tomato line Ty52 (homozygous for the Ty-1 allele for TYLCV tolerance) were exposed to viruliferous whiteflies (Bemisia tabaci Gennadius) in greenhouses at the Asian Vegetable Research and Development Center, Taiwan, and the University of Agricultural Sciences, Bangalore, India. Results indicated that F 3 families homozygous for the introgression on chromosome 11 were resistant to TYLCV at both locations. Additional probing showed that the chromosome 11 introgression spanned markers TG36 to TG393, covering a distance of at least 14.6 centimorgans. This is the first report of TYLCV resistance in tomato mapped to chromosome 11.

Subfields: Insect Science, Plant Science

View Paper: https://www.semanticscholar.org/paper/3ff20898b82c32efb97e4d7c4ff3ce3fddf4c508

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In Plant Science

1. John W. Scott
2. D. Maxwell
3. Yuanfu Ji
4. D. Schuster
5. C. Kole
6. T. Lumpkin
7. M. Foolad
8. K. Weinberger

Citations in the corpus

• Conventional and molecular marker-assisted selection and pyramiding of genes for multiple disease resistance in tomato (2016, background): “Seedling exposure to viruliferous whiteflies 6 Hanson et al. (2012)”
• Wild Crop Relatives: Genomic and Breeding Resources (2011, methodology): “Later the TYLCV resistance locus, originating from B6013, was mapped to the long arm of chromosome 11, using RFLP markers (Hanson et al. 2000), formally designated Ty-2, and further fine-mapped (Hanson et al.”
• Horticulture for poverty alleviation: the unfunded revolution (2005, background): “After only a few years ToLCV-resistant lines were developed that produce twice the yield of the most popular varieties in the region (Hanson et al., 2000).”
• Recessive Resistance to Tomato yellow leaf curl virus from the Tomato Cultivar Tyking Is Located in the Same Region as Ty-5 on Chromosome 4 (2012, background): “The single dominant genes Ty-1 and Ty-2 have failed in some production regions (Scott, 2007; Scott, personal communication).”
• Mapping QTLs conferring early blight (Alternaria solani) resistance in a Lycopersicon esculentum×L. hirsutum cross by selective genotyping (2003, background): “…Tanksley 1997); Tm-1 and Tm-2a, resistance to tobacco mosaic virus (Young and Tanksley 1989b); Ty-1 and Ty-2, resistance to yellow leaf curl virus (Hanson et al. 2000; Zamir et al. 1994); Ve, resistance to Verticillium dahliae (Diwan et al. 1999). allele frequency differences between the two…”
• Molecular Mapping of Ty-4, a New Tomato Yellow Leaf Curl Virus Resistance Locus on Chromosome 3 of Tomato (2009, background): “In addition, Ty-1 and Ty-2 genes express complete or nearly complete dominance (Hanson et al., 2000; Ji et al., 2009; Zamir et al., 1994), while Ty-3 is more additive (Ji et al., 2007a).”
• Ty-3, a begomovirus resistance locus near the Tomato yellow leaf curl virus resistance locus Ty-1 on chromosome 6 of tomato (2007, methodology): “…resistance have been developed from the aforementioned wild species (Zamir et al. 1994; Scott et al. 1995; Lapidot et al. 1997; Friedmann et al. 1998; Vidavsky and Czosnek 1998; Vidavsky et al. 1998; Hanson et al. 2000), and these are being widely used to breed resistant hybrids (Pico et al. 1999).”