Cell-laden microengineered gelatin methacrylate hydrogels

By J. Nichol, Sandeep T. Koshy, Hojae Bae, C. Hwang, Seda Yamanlar, Ali Khademhosseini. Biomaterials, 2010

2,190 citations recorded by the source corpus.

The cellular microenvironment plays an integral role in improving the function of microengineered tissues. Control of the microarchitecture in engineered tissues can be achieved through photopatterning of cell-laden hydrogels. However, despite high pattern fidelity of photopolymerizable hydrogels, many such materials are not cell-responsive and have limited biodegradability. Here we demonstrate gelatin methacrylate (GelMA) as an inexpensive, cell-responsive hydrogel platform for creating cell-laden microtissues and microfluidic devices. Cells readily bound to, proliferated, elongated and migrated both when seeded on micropatterned GelMA substrates as well as when encapsulated in microfabricated GelMA hydrogels. The hydration and mechanical properties of GelMA were demonstrated to be tunable for various applications through modification to the methacrylation degree and gel concentration. Pattern fidelity and resolution of GelMA was high and it could be patterned to create perfusable microfluidic channels. Furthermore, GelMA micropatterns could be used to create cellular micropatterns for in vitro cell studies or 3D microtissue fabrication. These data suggest that GelMA hydrogels could be useful for creating complex, cell-responsive microtissues, such as endothelialized microvasculature, or for other applications that requires cell-responsive microengineered hydrogels.

Subfields: Biomedical Engineering, Cell Biology

View Paper: https://www.semanticscholar.org/paper/3e3f155dc8347c803aae57c80b6fa2c291a63d04

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Citations in the corpus

Gelatin-Methacryloyl Hydrogels: Towards Biofabrication-Based Tissue Repair (2016, methodology): “The DoF of the synthesized gelMA batch can be tailored by varying the methacrylic anhydride:gelatin ratio [21,33].”
Synthesis, properties, and biomedical applications of gelatin methacryloyl (GelMA) hydrogels (2015, methodology): “Specifically, the RGD motifs do not contain groups that will react with MA, which ensures the retention of good cell adhesive properties of the GelMA [6, 19, 29].”
Functionalization, preparation and use of cell-laden gelatin methacryloyl–based hydrogels as modular tissue culture platforms (2016, background): “One cell-laden hydrogel is collected after days 1, 7 and 14 of 3D culture (or any other desired time point) for each replicate experiment.”
Hydrogel scaffolds for tissue engineering: Progress and challenges (2013, background): “In a recent study, Nichol et al.(29) have developed gelatin methacrylate (GelMA) as an inexpensive, cell-responsive hydrogel platform for creating cell-laden microtissues.”
Current advances and future perspectives in extrusion-based bioprinting. (2016, background): “Although hydrogels such as fibrin and GelMA support neovascularization, they may not provide the ideal microenvironment and signaling for survival, motility and differentiation of a wide array of tissue-specific cells, and their stability over prolonged in vitro culture is weak [216,217].”
A comprehensive review on droplet-based bioprinting: Past, present and future. (2016, background): “It has been used to engineer tissues including bone [61,88], cardiac [57], cartilage [33,35,59], liver [9,32], lung [91], neural tissue [34,81], skin [58,92,93], vascular tissue [67,94,95] and ovarian tumor tissue [38].”
Vascularization and Angiogenesis in Tissue Engineering: Beyond Creating Static Networks. (2016, background): “Individual channels [36–38] and interconnected channel networks [34,35,39,40] have been fabricated in hydrogels using sacrificial materials such as gelatin [39] or glass filaments [34] (Figure 1D), structures such as hypodermic needles that can be removed after gelation [37], or polydimethylsiloxane”
Current hydrogel advances in physicochemical and biological response-driven biomedical application diversity (2021, background): “, transglutaminase) cross-linking and photo-induced covalent cross-linking that correspond to pure and methacrylate-modified gelatin, respectively, are dominant.(79) Notably, the network architecture of gelatin and collagen hydrogel scaffolds are dramatically different, corresponding to macroporous ”