A Spatiotemporal Organ-Wide Gene Expression and Cell Atlas of the Developing Human Heart.

By Michaela Asp, Stefania Giacomello, L. Larsson, Chenglin Wu, Daniel Fürth, Xiaoyan Qian, E. Wärdell, Joaquín Custodio, Johan Reimegård, Fredrik Salmén and 10 more. Cell, 2019

670 citations recorded by the source corpus.

The process of cardiac morphogenesis in humans is incompletely understood. Its full characterization requires a deep exploration of the organ-wide orchestration of gene expression with a single-cell spatial resolution. Here, we present a molecular approach that reveals the comprehensive transcriptional landscape of cell types populating the embryonic heart at three developmental stages and that maps cell-type-specific gene expression to specific anatomical domains. Spatial transcriptomics identified unique gene profiles that correspond to distinct anatomical regions in each developmental stage. Human embryonic cardiac cell types identified by single-cell RNA sequencing confirmed and enriched the spatial annotation of embryonic cardiac gene expression. In situ sequencing was then used to refine these results and create a spatial subcellular map for the three developmental phases. Finally, we generated a publicly available web resource of the human developing heart to facilitate future studies on human cardiogenesis.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3e0e4ba5cb811a35d47450254c573b5aebc2dba2

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Citations in the corpus

Benchmarking spatial and single-cell transcriptomics integration methods for transcript distribution prediction and cell type deconvolution (2022, methodology): “We used the 16 integration methods to combine the spatial and single-cell transcriptomics data, and then compared their performance for (1) predicting the spatial distribution of RNA transcripts and (2) deconvoluting cell types of a histological spot.”
Spatiotemporal analysis of human intestinal development at single-cell resolution (2021, positive): “Spatial transcriptomics (ST) can map transcriptional signatures to distinct geographical regions that are vital in development, where patterning and location-specific morphogen gradients shape organogenesis (Asp et al., 2019).”