Spatiotemporal analysis of human intestinal development at single-cell resolution

By D. Fawkner-Corbett, A. Antanaviciute, K. Parikh, M. Jagielowicz, A. Gerós, Tarun Gupta, N. Ashley, Doran Khamis, Darren Fowler, Edward R. Morrissey and 4 more. Cell, 2021

423 citations recorded by the source corpus.

Summary Development of the human intestine is not well understood. Here, we link single-cell RNA sequencing and spatial transcriptomics to characterize intestinal morphogenesis through time. We identify 101 cell states including epithelial and mesenchymal progenitor populations and programs linked to key morphogenetic milestones. We describe principles of crypt-villus axis formation; neural, vascular, mesenchymal morphogenesis, and immune population of the developing gut. We identify the differentiation hierarchies of developing fibroblast and myofibroblast subtypes and describe diverse functions for these including as vascular niche cells. We pinpoint the origins of Peyer’s patches and gut-associated lymphoid tissue (GALT) and describe location-specific immune programs. We use our resource to present an unbiased analysis of morphogen gradients that direct sequential waves of cellular differentiation and define cells and locations linked to rare developmental intestinal disorders. We compile a publicly available online resource, spatio-temporal analysis resource of fetal intestinal development (STAR-FINDer), to facilitate further work.

Subfields: Molecular Biology, Oncology, Pulmonary and Respiratory Medicine

View Paper: https://www.semanticscholar.org/paper/feb8323b3f7f63b0a9dd773dd425e456ae56fe4c

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In Molecular Biology

  1. Roser Vento-Tormo
  2. Oliver Stegle
  3. E. Dann
  4. Moritz Gerstung
  5. Hamish W. King
  6. Adam Gayoso
  7. John C. Marioni

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