CDPKs CPK6 and CPK3 Function in ABA Regulation of Guard Cell S-Type Anion- and Ca2+- Permeable Channels and Stomatal Closure

By I. Mori, Y. Murata, Yingzhen Yang, S. Munemasa, Yong-Fei Wang, S. Andreoli, Hervé Tiriac, José M. Alonso, Jeffery F. Harper, Joseph R. Ecker and 2 more. PLoS Biology, 2006

637 citations recorded by the source corpus.

Abscisic acid (ABA) signal transduction has been proposed to utilize cytosolic Ca2+ in guard cell ion channel regulation. However, genetic mutants in Ca2+ sensors that impair guard cell or plant ion channel signaling responses have not been identified, and whether Ca2+-independent ABA signaling mechanisms suffice for a full response remains unclear. Calcium-dependent protein kinases (CDPKs) have been proposed to contribute to central signal transduction responses in plants. However, no Arabidopsis CDPK gene disruption mutant phenotype has been reported to date, likely due to overlapping redundancies in CDPKs. Two Arabidopsis guard cell–expressed CDPK genes, CPK3 and CPK6, showed gene disruption phenotypes. ABA and Ca2+ activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca2+-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca2+-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. However, rapid-type anion channel current activity was not affected, consistent with the partial stomatal closing response in double mutants via a proposed branched signaling network. Imposed Ca2+ oscillation experiments revealed that Ca2+-reactive stomatal closure was reduced in CDPK double mutant plants. However, long-lasting Ca2+-programmed stomatal closure was not impaired, providing genetic evidence for a functional separation of these two modes of Ca2+-induced stomatal closing. Our findings show important functions of the CPK6 and CPK3 CDPKs in guard cell ion channel regulation and provide genetic evidence for calcium sensors that transduce stomatal ABA signaling.

Subfields: Plant Science

View Paper: https://www.semanticscholar.org/paper/3b35ca320e70b34845180f69096cc23f0752fc9b

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Citations in the corpus

Abiotic and Biotic Stress Response Crosstalk in Plants (2011, background): “In other hand, CDPKs CPK6 and CPK3 operate in ABA regulation of guard cell S-type anion- and Ca2+- permeable channels and stomatal closure (Mori et al., 2006), but besides its well-established role in abiotic stress adaptation, recent results in rice plants indicate that ABA is also involved in the…”
Molecular Basis of the Core Regulatory Network in ABA Responses: Sensing, Signaling and Transport (2010, background): “Other CDPKs, CPK3 and CPK6, also function in the regulation of the anion channel in guard cells ( Mori et al. 2006 ).”
Plant abiotic stress response and nutrient use efficiency (2020, positive): “Some kinases, including OST1 (Geiger et al., 2009), CPKs/ CDPKs (CPK3, 6, 21, 23) (Brandt et al., 2012; Geiger et al., 2010; Maierhofer et al., 2014; Mori et al., 2006; Saito et al., 2018; Scherzer et al., 2012), and calcineurin-B like protein 5 (CBL5)–CBL-interacting protein kinase 11 (CIPK11)…”
Abiotic Stress Response in Plants - Physiological, Biochemical and Genetic Perspectives (2011, background): “In other hand, CDPKs CPK6 and CPK3 operate in ABA regulation of guard cell S-type anion- and Ca2+- permeable channels and stomatal closure (Mori et al., 2006), but besides its well-established role in abiotic stress adaptation, recent results in rice plants indicate that ABA is also involved in the…”
Guard Cell Signal Transduction Network: Advances in Understanding Abscisic Acid, CO2, and Ca2+ Signaling (2010, background): “The presence of functional redundancy of Ca2+-binding proteins (22, 120, 221) supports the observation of an overall robustness of the guard cell signaling network.”
Abscisic acid dynamics, signaling and functions in plants. (2019, background): “…phosphorylate and activate the Slow-type (S-type) anion efflux channels including SLOW ANION CHANNEL1 (SLAC1) and SLOW ANION CHANNEL-ASSOCIATED (SLAH3), which are involved in stomatal closure and reduction of leaf water loss (Mori et al. 2006; Zhu et al. 2007; Zou et al. 2010; Brandt et al. 2012).”
Calcium Signals: The Lead Currency of Plant Information Processing (2010, background): “long-term closure (Mori et al., 2006).”
New insights into plant responses to the attack from insect herbivores. (2010, background): “Several CDPKs in Arabidopsis are involved in abscisic acid (ABA) signaling and thus plant resistance to drought or salt stress (85, 149).”
Two Calcium-Dependent Protein Kinases, CPK4 and CPK11, Regulate Abscisic Acid Signal Transduction in Arabidopsis[W] (2007, background): “…and abiotic stresses (Bachmann et al., 1995, 1996; McMichael et al., 1995; Pei et al., 1996; Sheen, 1996; Li et al., 1998; Sugiyama et al., 2000; Romeis et al., 2001; Hrabak et al., 2003; Shao and Harmon, 2003; McCubbin et al., 2004; Choi et al., 2005; Ivashuta et al., 2005; Mori et al., 2006).”