A practical guide to single-molecule FRET

By R. Roy, S. Hohng, T. Ha. Nature Methods, 2008

2,046 citations recorded by the source corpus.

Subfields: Biophysics, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3a7c54aab5302a6a92cf109d76437f92a4ff8363

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Citations in the corpus

Photophysics of Fluorescence Probes for Single Molecule Biophysics and Super-Resolution Imaging (2012, positive): “Another approach is FRET where the colocalization of two differently labeled molecules is indicated by the occurrence of FRET and the efficiency of FRET reports on the distance between a donor and an acceptor fluorophore(14).”
High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence (2013, background): “However, these traditional singlemolecule methods are hindered by limitations in maximal fluorescent component concentrations (up to 50 nM) (1), limited simultaneous detection (two to three colors) (2–6), and low throughput (a few hundred molecules at most per experiment) (7).”
Nanoantenna-enhanced gas sensing in a single tailored nanofocus (2011, background): “Our work will advance the field of catalytic-activity studies, which largely rely on fluorescence techniques”
Recent advances in RNA structurome (2022, methodology): “FRET makes use of the mechanism of “Förster Resonance Energy Transfer” to transfer energy from donor to acceptor fluorophores (Roy et al., 2008).”
SLC6 Neurotransmitter Transporters: Structure, Function, and Regulation (2011, methodology): “Singlemolecule fluorescence resonance energy transfer (FRET) (Roy et al., 2008) was used to measure time-resolved dynamic behavior of the intracellular gating region in single LeuT molecules during the application of substrates and inhibitors.”
High-resolution atomic force microscopy and spectroscopy of native membrane proteins (2011, positive): “In the future, sophisticated approaches such as single-molecule FRET [382, 383], confocal microscopy, or even super-resolution fluorescence microscopy [384, 385] will be increasingly combined with AFM-based methods.”
Dynamics of proteins in solution (2019, background): “…exhibits a strong distance dependence on the scale of a few nanometers, which allows for high-precision measurements of the distance between labeled sites by measuring the efficiency of the transfer (Deniz et al., 2001; Lippincott-Schwartz et al., 2001; Piston and Kremers, 2007; Roy et al., 2008).”
Learning rates and states from biophysical time series: a Bayesian approach to model selection and single-molecule FRET data. (2009, background): “Biophysical Journal 97(12) 3196–3205 open source, including a point and click GUI.”
The Influenza A Virus PB2, PA, NP, and M Segments Play a Pivotal Role during Genome Packaging (2012, methodology): “Single-molecule imaging was performed using a prism-type total internal reflection fluorescence (TIRF) microscope, and the single-molecule analysis was performed as described previously (16, 32).”