Characterization and direct quantitation of cerebroside molecular species from lipid extracts by shotgun lipidomics Published, JLR Papers in Press, October 16, 2004. DOI 10.1194/jlr.D400022-JLR200
By Xianlin Han, H. Cheng. 2005
117 citations recorded by the source corpus.
By using shotgun lipidomics based on the separation of lipid classes in the electrospray ion source (intrasource separation) and two-dimensional (2D) MS techniques (Han, X., and R. W. Gross. 2004. Shotgun lipidomics: electrospray ionization mass spectrometric analysis and quantitation of the cellular lipidomes directly from crude extracts of biological samples. Mass Spectrom. Rev. First published on June 18, 2004; doi: 10.1002/mas.20023, In press), individual molecular species of most major and many minor lipid classes can be quantitated directly from biological lipid extracts. Herein, we extended shotgun lipidomics to the characterization and quantitation of cerebroside molecular species in biological samples. By exploiting the differential fragmentation patterns of chlorine adducts using electrospray ionization (ESI) tandem mass spectrometry, hydroxy and nonhydroxy cerebroside species are readily identified. The hexose (either galactose or glucose) moiety of a cerebroside species can be distinguished by examination of the peak intensity ratio of its product ions at m/z 179 and 89 (i.e., 0.74 ± 0.10 and 4.8 ± 0.7 for galactose- and glucose-containing cerebroside species, respectively). Quantitation of cerebroside molecular species (as little as 10 fmol) from chloroform extracts of brain tissue samples was directly conducted by 2D ESI/MS after correction for differences in 13C-isotopomer intensities. This method was demonstrated to have a greater than 1,000-fold linear dynamic range in the low concentration region; therefore, it should have a wide range of applications in studies of the cellular sphingolipid lipidome.
Subfields: Molecular Biology, Spectroscopy
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- Mammalian Ceramide Synthases (2010, background): “CerS3 is able to synthesize ceramides containing a-hydroxy (2-hydroxy) fatty acids, which are abundant in the skin (65, 66) where they are involved in maintaining the water permeability barrier function (67).”