Live tissue intrinsic emission microscopy using multiphoton-excited native fluorescence and second harmonic generation

By W. Zipfel, Rebecca M. Williams, R. Christie, A. Nikitin, Bradley T. Hyman, W. Webb. Proceedings of the National Academy of Sciences of the United States of America, 2003

1,804 citations recorded by the source corpus.

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

Intravital imaging of cell movement in tumours (2003, background): “ECM fibres that are composed of collagen and possibly other ECM proteins are identifiable and migration of cancer cells is most evident on these long ECM fibre”
Interpreting second-harmonic generation images of collagen I fibrils. (2005, methodology): “Fits to exact field calculations (Richards andWolf, 1959) yield explicit values for the size of the focal volume (Zipfel et al., 2003b): w ¼ 0:32lv nvsinQ and wz ¼ 0:53lv nvð1 cosQÞ ; (6) where nvsinQ is the N.A. of the microscope objective.”
Optical coherence tomography today: speed, contrast, and multimodality (2014, neutral): “Label-free MPM is also possible via three-photon processes and can be used to image tryptophan and serotonin.(176) Unlike TPF, SHG is a parametric process which does not involve photon absorption and emission.”
Collagen reorganization at the tumor-stromal interface facilitates local invasion (2006, background): “Combined MPE-SHG produced clear images of collagen (Additional file 1) as well as endogenous fluorophores, such as NAD(H) and FAD (Additional file 1, part C), in intact non-treated tissues [13,15,21].”
Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure (2012, background): “Calculating the anisotropy parameter β with equation (4) yielded a value of ~ 0.”