BS-Seeker2: a versatile aligning pipeline for bisulfite sequencing data
By Weilong Guo, P. Fiziev, Weihong Yan, Shawn Cokus, Xueguang Sun, Michael Q. Zhang, Pao-Yang Chen, Matteo Pellegrini. BMC Genomics, 2013
400 citations recorded by the source corpus.
DNA methylation is an important epigenetic modification involved in many biological processes. Bisulfite treatment coupled with high-throughput sequencing provides an effective approach for studying genome-wide DNA methylation at base resolution. Libraries such as whole genome bisulfite sequencing (WGBS) and reduced represented bisulfite sequencing (RRBS) are widely used for generating DNA methylomes, demanding efficient and versatile tools for aligning bisulfite sequencing data. We have developed BS-Seeker2, an updated version of BS Seeker, as a full pipeline for mapping bisulfite sequencing data and generating DNA methylomes. BS-Seeker2 improves mappability over existing aligners by using local alignment. It can also map reads from RRBS library by building special indexes with improved efficiency and accuracy. Moreover, BS-Seeker2 provides additional function for filtering out reads with incomplete bisulfite conversion, which is useful in minimizing the overestimation of DNA methylation levels. We also defined CGmap and ATCGmap file formats for full representations of DNA methylomes, as part of the outputs of BS-Seeker2 pipeline together with BAM and WIG files. Our evaluations on the performance show that BS-Seeker2 works efficiently and accurately for both WGBS data and RRBS data. BS-Seeker2 is freely available at http://pellegrini.mcdb.ucla.edu/BS_Seeker2/ and the Galaxy server.
Subfields: Genetics, Molecular Biology
View Paper: https://www.semanticscholar.org/paper/39087c9b3085cea370f5b18ba259da7c8e42e0e0
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Citations in the corpus
- Next-Generation Sequencing Technology: Current Trends and Advancements (2023, background): “Alignment of reads to reference Splice-aware aligner such as TopHat2 [123], HISAT2 [124], and STAR [125] Transcript quantification featureCounts [126], HTSeq-count [127], Salmon [128], Kallisto [129] Differential gene expression analysis enrichment of gene categories DESeq2 [130], EdgeR [131], DAVID ”