Regulation of the Arabidopsis transcriptome by oxidative stress.

By Radhika Desikan, S. A.‐H.‐Mackerness, J. Hancock, S. Neill. Plant Physiology, 2001

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Oxidative stress, resulting from an imbalance in the accumulation and removal of reactive oxygen species such as hydrogen peroxide (H(2)O(2)), is a challenge faced by all aerobic organisms. In plants, exposure to various abiotic and biotic stresses results in accumulation of H(2)O(2) and oxidative stress. Increasing evidence indicates that H(2)O(2) functions as a stress signal in plants, mediating adaptive responses to various stresses. To analyze cellular responses to H(2)O(2), we have undertaken a large-scale analysis of the Arabidopsis transcriptome during oxidative stress. Using cDNA microarray technology, we identified 175 non-redundant expressed sequence tags that are regulated by H(2)O(2). Of these, 113 are induced and 62 are repressed by H(2)O(2). A substantial proportion of these expressed sequence tags have predicted functions in cell rescue and defense processes. RNA-blot analyses of selected genes were used to verify the microarray data and extend them to demonstrate that other stresses such as wilting, UV irradiation, and elicitor challenge also induce the expression of many of these genes, both independently of, and, in some cases, via H(2)O(2).

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/384058d20760cb28ab8eab31a391db171aafdbfc

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Citations in the corpus

Ascorbate Peroxidase and Catalase Activities and Their Genetic Regulation in Plants Subjected to Drought and Salinity Stresses (2015, background): “A study conducted by using DNA microarray technology demonstrated that cells of Arabidopsis exposed to H2O2 changed the expression levels of 175 genes, and 113 genes were induced to encode for proteins acting as antioxidants, or as stress defense responses, or as signaling proteins [82].”
Oxidative stress and ozone: perception, signalling and response (2005, neutral): “plants (Desikan et al. 2001; Tamaoki et al. 2003a) and the”
THE ROLE OF CALCIUM IN PLANTS' SALT TOLERANCE (2012, background): “In glycophytes, salinity imposes ionic stress (Munns and Tester, 2008), osmotic stress (Thiyagarajah et al., 1996) and secondary stresses such as nutritional disorders (Forde et al., 2004) and oxidative stresses (Desikan et al., 2001).”
Changes in activities of antioxidant enzymes and lipoxygenase during growth of sunflower seedlings from seeds of different vigour (2002, background): “Finally, the regulation of H2O2 homeostasis by CAT could be important in terms of control of the H2O2-dependent signalling pathway, which has been shown to involve many genes, including that of LOX (Desikan et al., 2001).”
Peroxisomes and oxidative stress. (2006, background): “, wounding, pathogen attack, drought, osmotic stress, excess light) that generate H2O2 as a signalling molecule result in peroxisome proliferation via the up-regulation of PEX genes required for peroxisome biogenesis and import of proteins [27,105,106].”
Involvement of soluble sugars in reactive oxygen species balance and responses to oxidative stress in plants. (2006, background): “, 2004) and the oxidative-stress transcriptome (Desikan et al., 2001; Rossel et al., 2002) in order to con-”
Cyanide action in plants — from toxic to regulatory (2006, background): “More over, a se quence sim i lar to the NF-KB rec og ni tion site was iden ti fied in the pro mot ers of sev eral plant de fense genes (Desikan et al. 2001, Vranova et al. 2002).”
Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis (2004, background): “In contrast to the PR-1 regulon consisting of 26 coordinated expressed genes which all contained W-boxes in their promoters (Maleck et al., 2000), no common cis-element could be identified in 1.1 kbp upstream region of the hydrogen peroxide ESTs (Desikan et al., 2001).”
Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, neutral): “Utilization of genome-wide approaches demonstrated that expression-level effects of xenobiotics in plants were large-scale and coordinated, thus resulting in xenobiotic-response gene networks involving induction and repression of hundreds of genes, as is the case for other abiotic stress responses (”
Hydrogen peroxide and nitric oxide as signalling molecules in plants. (2002, methodology): “This work has been extended to a microarray analysis using the Arabidopsis Functional Genomics Facility (AFGC, Desikan et al., 2001a).”
Sensor types in signal transduction pathways in plant cells responding to abiotic stressors: do they depend on stress intensity? (2004, background): “tures, excess excitation energy, ultraviolet (UV) irradiation and ozone (Desikan et al. 2001), and may constitute the basis for the phenomenon of cross-tolerance, in”