Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation.

By M. Betts, J. Brenchley, D. Price, S. De Rosa, Daniel C. Douek, Mario Roederer, R. Koup. JIM - Journal of Immunological Methods, 2003

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Subfields: Immunology

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T-cell quality in memory and protection: implications for vaccine design (2008, background): “Measuring these directly (or by using CD107 as an indirect measure of degranulation) provides further insight into important effector pathway”
Tumor-derived granulocyte-macrophage colony stimulating factor regulates myeloid inflammation and T cell immunity in pancreatic cancer (2012, background): “To understand if CD8 T cells from GM-CSF knockdown tumors exhibited effector functions, we evaluated tumor-infiltrating CD8 T cells for their capacity to secrete IFN-g and mobilize CD107a, the latter serving as a biomarker of lytic function (Betts et al., 2003).”
Immunopathology of autoantibody-associated encephalitides: clues for pathogenesis. (2012, methodology): “These findings mirror the stages of GrB-mediated cytotoxic T cell attack documented in vitro (Hahn et al., 1994; Betts et al., 2003).”