Targets of AtWRKY6 regulation during plant senescence and pathogen defense.

By S. Robatzek, I. Somssich. Genes & Development, 2002

654 citations recorded by the source corpus.

In Arabidopsis, WRKY factors comprise a large gene family of plant-specific transcriptional regulators controlling several types of plant stress responses. To understand the regulatory role of WRKY proteins during such processes, we identified targets of the senescence- and defense-associated WRKY6 factor. WRKY6 was found to suppress its own promoter activity as well as that of a closely related WRKY family member, indicating negative autoregulation. On the other hand, WRKY6 positively influenced the senescence- and pathogen defense-associated PR1 promoter activity, most likely involving NPR1 function. One novel identified target gene, SIRK, encodes a receptor-like protein kinase, whose developmental expression is strongly induced specifically during leaf senescence. The transcriptional activation of SIRK is dependent on WRKY6 function. Senescing leaves of wrky6 knockout mutants showed a drastic reduction, and green leaves of WRKY6 overexpression lines showed clearly elevated SIRK transcript levels. Furthermore, the SIRK gene promoter was specifically activated by WRKY6 in vivo, functioning very likely through direct W-box interactions.

Subfields: Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/37580047d56ff058985ea63e74c0c1f8a6459e8b

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In Molecular Biology

1. A. Fischer
2. Cyril Zipfel
3. G. Felix
4. Jonathan D. G. Jones
5. Diqiu Yu
6. T. Boller
7. L. Navarro
8. Yuping Qiu

In Plant Science

1. Cyril Zipfel
2. T. Boller
3. A. Fischer
4. Jonathan D. G. Jones
5. G. Felix
6. S. Robatzek
7. Diqiu Yu
8. Karam B. Singh

Citations in the corpus

• The N Terminus of Bacterial Elongation Factor Tu Elicits Innate Immunity in Arabidopsis Plants (2004, methodology): “Induction of the SIRK/FRK1 gene (At2g19190) has been used in several studies as a molecular marker for induction of defenserelated genes during basal defense (Asai et al., 2002; Robatzek and Somssich, 2002; de Torres et al., 2003).”
• Bacterial disease resistance in Arabidopsis through flagellin perception (2004, methodology): “For control transformation, a SIRKp::GUS construc”
• The WRKY Transcription Factor Family in Model Plants and Crops (2017, background): “AtWRKY6 binds to a receptor-like kinase Senescenceinduced receptor-like serine/threonine-protein kinase and regulates the leaf senescence process (Robatzek and Somssich, 2002).”
• Targets of the WRKY53 transcription factor and its role during leaf senescence in Arabidopsis (2004, background): “Since no significant overlap to the target genes already characterized for WRKY6 or WRKY70 (Robatzek and Somssich, 2002; Li et al., 2004) was observed, this methods appears to be suitable to identify specific target genes of WRKY53.”
• The Complex Regulation of Senescence (2012, background): “Cross-talk between defense and senescence signaling is a recurrent subject in the senescence literature, and WRKY genes/proteins are of particular importance in this context (e.g. Robatzek and Somssich, 2002; Wingler and Roitsch, 2008; Rushton et al., 2010).”
• The molecular analysis of leaf senescence--a genomics approach. (2002, background): “A senescence specific receptor kinase gene, SIRK, that was dependent on WRKY6 for expression was also identified (Robatzek and Somssich, 2002).”
• Over-expression of the stress-induced OsWRKY45 enhances disease resistance and drought tolerance in Arabidopsis (2009, background): “…in the regulation of various response processes, including pathogen infection (Eulgem et al., 1999; Chen and Chen, 2000, 2002; Du and Chen, 2000; Robatzek and Somssich, 2001, 2002; Yu et al., 2001; Cormack et al., 2002; Yoda et al., 2002; Dong et al., 2003; Kalde et al., 2003; Turck et al.,…”
• The ectopic expression of the rice Osmyb4 gene in Arabidopsis increases tolerance to abiotic, environmental and biotic stresses (2006, background): “Among them, a set of transcription factor encoding genes was induced, such as AtERF6, EREBP4 and WRKY6 [43–45].”
• Cloning and expression profiles of 15 genes encoding WRKY transcription factor in wheat (Triticum aestivem L.) (2008, background): “It was demonstrated that WRKY transcriptional factors played a key role in leaf senescence [37–39].”
• The role of WRKY transcription factors in plant abiotic stresses. (2012, background): “For example, several downstream genes, including FRK1/SIRK, were identified to be AtWRKY6's target gene, and these genes work together to play important roles in the plant leaf senescence [87].”
• The WRKY Gene Family in Rice (Oryza sativa) (2007, background): “Similarly, Arabidopsis AtWRKY6 negatively autoregulates its own promoter while transcriptionally activating SIRK (Robatzek and Somssich 2002).”
• The WRKY70 Transcription Factor: A Node of Convergence for Jasmonate-Mediated and Salicylate-Mediated Signals in Plant Defense On-line version contains Web-only data. (2004, background): “…factors have been shown to confer disease resistance (Deslandes et al., 2002), trigger expression of defense-related genes in SAR (Eulgem et al., 1999; Maleck et al., 2000; Robatzek and Somssich, 2002), and control A. thaliana innate immunity activated by bacterial flagellin (Asai et al., 2002).”
• The Transcriptional Innate Immune Response to flg22. Interplay and Overlap with Avr Gene-Dependent Defense Responses and Bacterial Pathogenesis1[w] (2004, background): “Among those, we identified AtWRKY6 (At1g62300; Robatzek and Somssich, 2002) as well as AtWRKY22 and AtWRKY29 (At4g01250 and At4g23550), whose overexpression increased resistance to both bacterial and fungal pathogens (Asai et al., 2002).”