A guide to genome engineering with programmable nucleases
By H. Kim, Jin-Soo Kim. Nature reviews genetics, 2014
1,115 citations recorded by the source corpus.
Subfields: Business and International Management, Molecular Biology, Plant Science
View Paper: https://www.semanticscholar.org/paper/3744283196d8c1b423f5552894bc4453a4c302b6
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Citations in the corpus
- Cas-analyzer: an online tool for assessing genome editing results using NGS data (2016, background): “Programmable nucleases such as zinc finger nucleases (ZFNs), transcription-activator-like effector nucleases (TALENs), and RNAguided endonucleases derived from CRISPR-Cas9/Cpf1 systems, which are adaptive immune responses in bacteria and archaea, are widely used for genome editing in many research f”
- Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases (2014, methodology): “Aligned reads were filtered on the following conditions: (1) reads must include both a bait alignment and a prey alignment and (2) the bait alignment cannot extend more than 10 nucleotides beyond the targeted site.”
- Highly efficient RNA-guided genome editing in human cells via delivery of purified Cas9 ribonucleoproteins (2014, methodology): “We have developed and improved all of these types of programmable nucleases over the last several years (Kim et al. 2009, 2010, 2011; Cho et al. 2013a; Kim et al. 2013a; Sung et al. 2013) and reported that the specificity and activity of RGENs are at least on par with those of their precursors.”
- Easy quantitative assessment of genome editing by sequence trace decomposition (2014, methodology): “(b) Overview of TIDE algorithm and output, which consists of three main steps: (1) Visualization of aberrant sequence signal in control (black) and treated sample (green), the expected break site (vertical dotted line) and the region used for decomposition (gray bar); (2) Decomposition yielding the ”
- Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew (2014, neutral): “Sequence-specific nucleases have been applied to engineer targeted modifications in polyploid genomes 1 , but simultaneous modification of multiple homoeoalleles has not been reported.”
- Applications of genome editing technology in the targeted therapy of human diseases: mechanisms, advances and prospects (2020, background): “Thus, similar to RNAi, NHEJ may be applied in immortalized cell lines to generate the inactivation of a single gene or multiple genes, but by creating loss-of-function mutations, it may lead to permanent gene inactivation.(9) In the early development stage of genome editing, to induce the desired DS”