The spliceosome deposits multiple proteins 20–24 nucleotides upstream of mRNA exon–exon junctions

By Hervé Le Hir, Elisa Izaurralde, L. Maquat, Melissa J. Moore. EMBO Journal, 2000

987 citations recorded by the source corpus.

Subfields: Molecular Biology

View Paper: https://www.semanticscholar.org/paper/3551d43b73dadc93f3784645816b31436145a037

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In Molecular Biology

1. O. Isken
2. V. Narry Kim
3. A. Yamashita
4. Jack D. Keene
5. S. Ohno
6. Matthias W. Hentze
7. S. Hoshino
8. N. Kataoka

Citations in the corpus

• RNA regulons: coordination of post-transcriptional events (2007, background): “2a ), where their stability and translation are regulate”
• Messenger-RNA-binding proteins and the messages they carry (2002, background): “Further studies identified further protein components that bind mRNAs produced by splicing, and showed that they are organized into a complex near exon?exon junctions (∼20 nucleotides upstream of the junctions) in vitro and and in vivo”
• Nonsense-mediated mRNA decay: splicing, translation and mRNP dynamics (2004, background): “Each EJC that assembles during pre-mRNA splicing in HeLa-cell nuclear extracts protects only 8–10 nt of spliced product from RNase digestio”
• Quality control of eukaryotic mRNA: safeguarding cells from abnormal mRNA function. (2007, background): “Generally, NMD targets are recognized depending on a post-splicing exon junction complex (EJC) of proteins that is deposited ∼20–24 nucleotides (nt) upstream of exon–exon junctions (Le Hir et al. 2000, 2001b).”
• Binding of a novel SMG-1-Upf1-eRF1-eRF3 complex (SURF) to the exon junction complex triggers Upf1 phosphorylation and nonsense-mediated mRNA decay. (2006, background): “The positional information that marks exon–exon junctions is provided by the components of the splicing-dependent exon junction complex (EJC) that persist during export and until the mRNA is translated (Kataoka et al. 2000; Le Hir et al. 2000; 6These authors contributed equally to this work.”
• Nonsense-mediated decay approaches the clinic (2004, background): “This seems to occur through splicing-mediated marking of exon-exon boundaries, in which a dynamic assembly of proteins, known as the exon junction complex (EJC; Table 1 ), is deposited in a sequence-nonspecific manner ∼20–24 nucleotides 5′ to every exon-exon junctio”