Self-organisation of the human embryo in the absence of maternal tissues
By M. Shahbazi, A. Jędrusik, S. Vuoristo, G. Recher, A. Hupalowska, V. Bolton, N. Fogarty, A. Campbell, L. Devito, Dusko Ilic and 4 more. Nature Cell Biology, 2016
613 citations recorded by the source corpus.
Remodelling of the human embryo at implantation is indispensable for successful pregnancy. Yet it has remained mysterious because of the experimental hurdles that beset the study of this developmental phase. Here, we establish an in vitro system to culture human embryos through implantation stages in the absence of maternal tissues and reveal the key events of early human morphogenesis. These include segregation of the pluripotent embryonic and extra-embryonic lineages, and morphogenetic rearrangements leading to generation of a bilaminar disc, formation of a pro-amniotic cavity within the embryonic lineage, appearance of the prospective yolk sac, and trophoblast differentiation. Using human embryos and human pluripotent stem cells, we show that the reorganization of the embryonic lineage is mediated by cellular polarization leading to cavity formation. Together, our results indicate that the critical remodelling events at this stage of human development are embryo-autonomous, highlighting the remarkable and unanticipated self-organizing properties of human embryos.
Subfields: Molecular Biology, Public Health, Environmental and Occupational Health
View Paper: https://www.semanticscholar.org/paper/354074e0941ca9bab2df07fccf2c6c1540e3fd2e
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In Molecular Biology
Citations in the corpus
- Proliferation, survival and metabolism: the role of PI3K/AKT/mTOR signalling in pluripotency and cell fate determination (2016, background): “Equally, recent advancements in the ability to observe human development in vitro for extended periods beyond implantation (Deglincerti et al., 2016; Shahbazi et al., 2016) and single-cell gene expression profiling (Petropoulos et al.”