Determination of bacterial load by real-time PCR using a broad-range (universal) probe and primers set.

By M. Nadkarni, F. E. Martin, N. A. Jacques, N. Hunter. Microbiology, 2002

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Using the class 1 integron-integrase gene as a proxy for anthropogenic pollution (2014, positive): “Primers for amplification of 16S rRNA genes should be specific for bacteria (Nadkarni et al., 2002).”
Group-specific primer and probe sets to detect methanogenic communities using quantitative real-time polymerase chain reaction. (2005, background): “, 2001) allows rapid, highly sensitive detection of microbial DNA, thus indicating the presence of a target microorganism or group of microorganisms (Nadkarni et al., 2002; Suzuki et al., 2000).”
Quantitative PCR provides a simple and accessible method for quantitative microbiota profiling (2020, methodology): “In this study, we chose a widely-used universal bacterial primer set optimized for qPCR [29] that similarly targets the V3-V4 hypervariable regions as the primer set used for Illumina MiSeq (S1A Table).”
Microbial Community Analysis of Opalinus Clay Drill Core Samples from the Mont Terri Underground Research Laboratory, Switzerland (2007, methodology): “Quantitative real-time PCR (ABI Prism 7000 or 7700, Applied Biosystems) was used to determine the 16S-rDNA copy numbers of total Bacteria ( Nadkarni et al. 2002; Schippers et al.”