Genome sequence of Aedes aegypti, a major arbovirus vector

By S. Sinkins. Science, 2007

1,144 citations recorded by the source corpus.

We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae. Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster. Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.

Subfields: Infectious Diseases, Insect Science, Public Health, Environmental and Occupational Health

View Paper: https://www.semanticscholar.org/paper/2fbbd2428577942d9e501810dce74060d09ce36f

How researchers see this paper

The researchers whose trusted networks rank it highest, strongest advocate first. Every researcher gets a different order.

In Infectious Diseases

1. Y. Surget-Groba
2. J. Montoya-Burgos
3. G. Dimopoulos
4. B. Greenwood
5. Owen White
6. P. Alonso
7. E. Harris
8. Wei Zhu

In Insect Science

1. G. Dimopoulos
2. Y. Surget-Groba
3. J. Montoya-Burgos
4. B. Greenwood
5. Owen White
6. P. Alonso
7. E. Harris
8. Wei Zhu

In Public Health, Environmental and Occupational Health

1. G. Dimopoulos
2. Y. Surget-Groba
3. J. Montoya-Burgos
4. B. Greenwood
5. Owen White
6. P. Alonso
7. E. Harris
8. Wei Zhu

Citations in the corpus

• Global spread and persistence of dengue. (2008, background): “aegypti genome (94) will facilitate identification of genes linked to previously described QTLs associated, for example, with midgut and other barriers to infection.”
• Malaria: progress, perils, and prospects for eradication. (2008, positive): “Broad-based analyses of the genomes of vectors (130, 131) and pathogens, and the use of these new genomic data to better understand the complex population structure of natural vector populations, hold the key to long-term solutions.”
• Automated eukaryotic gene structure annotation using EVidenceModeler and the Program to Assemble Spliced Alignments (2007, methodology): “EVM was heavily utilized for the genome analysis of the mosquito Aedes aegypti [39], and used partially or exclusively to generate the preliminary annotation for recently sequenced genomes of the blood fluke Schistosoma mansoni [40], the protozoan oyster parasite Perkinsus marinus, the human body lo”
• The Aedes aegypti Toll Pathway Controls Dengue Virus Infection (2008, methodology): “The expression data were processed and analyzed as described previously [28].”
• Optimization of de novo transcriptome assembly from next-generation sequencing data. (2010, methodology): “We investigated the performances of the two alternative methods using the transcriptome of Ae. aegypti (Gibbons et al. 2009), for which the complete genome is known (Nene et al. 2007).”
• De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds (2016, methodology): “Below the histogram tracks we show the linkage groups reported in AaegL2 (18).”