Antioxidant and cryoprotective effects of a tetrapeptide isolated from Amur sturgeon skin gelatin

By M. Nikoo, S. Benjakul, A. Ehsani, L. Jing, Fengfeng Wu, N. Yang, Baocai Xu, Zhengyu Jin, Xueming Xu. 2014

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Abstract The peptide Pro–Ala–Gly–Tyr (PAGT) was isolated from Amur sturgeon skin gelatin and its antioxidant activity and cryoprotective effect in Japanese sea bass ( Lateolabrax japonicus ) mince was investigated. PAGT showed scavenging activity against DPPH, ABTS and hydroxyl radicals with no metal chelating activity. At a level of 25 ppm, PAGT prevented lipid oxidation in minced fish, but at higher levels it was ineffective. Low-field nuclear magnetic resonance (LF 1 H NMR) detected three water pools in mince in the range of 1–10, 10–100 and 100–300 microsecond (ms), respectively. PAGT influenced water distribution in the mince. Mince with PAGT (25 ppm) had the highest T 21 population, corresponding to a lower amount of water in T 22 population after 6 freeze/thaw cycles ( P

Subfields: Animal Science and Zoology, Molecular Biology, Physiology

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