Replication of Norovirus in Cell Culture Reveals a Tropism for Dendritic Cells and Macrophages
By C. Wobus, Stephanie M. Karst, Larissa B. Thackray, Kyeong-Ok Chang, S. Sosnovtsev, G. Belliot, A. Krug, J. Mackenzie, K. Green, Herbert W. Virgin. PLoS Biology, 2004
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Noroviruses are understudied because these important enteric pathogens have not been cultured to date. We found that the norovirus murine norovirus 1 (MNV-1) infects macrophage-like cells in vivo and replicates in cultured primary dendritic cells and macrophages. MNV-1 growth was inhibited by the interferon-αβ receptor and STAT-1, and was associated with extensive rearrangements of intracellular membranes. An amino acid substitution in the capsid protein of serially passaged MNV-1 was associated with virulence attenuation in vivo. This is the first report of replication of a norovirus in cell culture. The capacity of MNV-1 to replicate in a STAT-1-regulated fashion and the unexpected tropism of a norovirus for cells of the hematopoietic lineage provide important insights into norovirus biology.
Subfields: Cardiology and Cardiovascular Medicine, Genetics, Infectious Diseases
View Paper: https://www.semanticscholar.org/paper/2f8db53bcc132c6c79d8f95cf03d868ebf642781
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Citations in the corpus
- Norovirus classification and proposed strain nomenclature. (2006, background): “The murine strain, MNV-1, is the only NoV that grew successfully in cell culture and in a small animal model (Karst et al., 2003; Wobus et al., 2004).”