Determination of the molecular weight of proteins in solution from a single small-angle X-ray scattering measurement on a relative scale

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BioXTAS RAW: improvements to a free open-source program for small-angle X-ray scattering data reduction and analysis (2017, methodology): “…known standard, (ii) the absolute intensity value of I(0) (Orthaber et al., 2000; Mylonas & Svergun, 2007), (iii) the adjusted Porod volume method (Fischer et al., 2010) and (iv) the volume of correlation method (Rambo & Tainer, 2013). xS1 in the supporting information describes the validation of…”
Laplace Variational Approximation for Semiparametric Regression in the Presence of Heteroskedastic Errors (2016, background): “Here we consider estimation of the radius of gyration,R2 g = −3β2, and the intercept of the log intensity curve, β0, which is known to be linearly related to log molecular weight, given fixed concentration and other experimental parameters (Fischer et al. 2009).”
New developments in the ATSAS program package for small-angle scattering data analysis (2012, positive): “Recently, a web portal ‘SAXS MoW’ was introduced (Fischer et al., 2010), which employs the Porod volume to obtain an assessment of MM.”
ATSAS 2.8: a comprehensive data analysis suite for small-angle scattering from macromolecular solutions (2017, methodology): “DATMOW and DATVC are implementations of the SAXS MoW MW estimation as originally described by Fischer et al. (2010) and the volume of correlation (Vc) MW estimate by Rambo & Tainer (2013), respectively.”