Development of cryopreservation techniques.

By A. Sakai, F. Engelmann, H. Takagi. 2000

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Challenges in implementing plant shoot tip cryopreservation technologies (2020, background): “In addition, the age of the culture may play a significant role in the success of the procedure (Normah et al. 2019; Popova et al. 2015; Volk and Walters 2003; Benson 2008; Sakai 2000).”
Plant cryopreservation: Progress and prospects (2004, methodology): “It has been applied to apices of an increasing number of species (Sakai, 2000; Sakai et al., 2002).”
Development and large scale application of cryopreservation techniques for shoot and somatic embryo cultures of tropical crops (2007, background): “Vitrification and non-crystalline solid formation Vitrification is the freeze-avoidance mechanism that enables hydrated cells, tissues and organs to withstand exposure to the temperature of liquid nitrogen (Sakai 2000).”
Status of cryopreservation technologies in plants (crops and forest trees). (2005, methodology): “Recently, a new technique has been proposed, named “encapsulation/vitrification” [51].”