Status of cryopreservation technologies in plants (crops and forest trees).

By B. Panis, M. Lambardi, J. Ruane, A. Sonnino. 2005

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Advances in cryopreservation of in vitro-derived propagules: technologies and explant sources (2020, background): “2019), and forest species (Panis and Lambardi 2006; Li et al.”
Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation (2009, background): “…cryogenic techniques have been successfully applied to a large number of plant species including agricultural and horticultural crops from temperate and tropical regions (Reed, 2002; Lambardi & De Carlo, 2003; Engelmann, 2004; Panis & Lambardi, 2006; Wang & Perl, 2006; Gonzalez-Arnao et al., 2008).”
Current Issues in Plant Cryopreservation (2012, methodology): “…of this method include avoiding the use of high concentrations of (potentially toxic) CPAs (Reinhoud et al., 2000) and the presence of a nutritive bead, which may enhance postregeneration survival or re-growth of the material following immersion in LN and rewarming (Panis & Lambardi, 2005).”