Overexpression of the Bt cry2Aa2 operon in chloroplasts leads to formation of insecticidal crystals

By Brandy De Cosa, W. Moar, Seung-Bum Lee, Michael Miller, H. Daniell. Nature Biotechnology, 2001

594 citations recorded by the source corpus.

Subfields: Biotechnology, Molecular Biology, Plant Science

View Paper: https://www.semanticscholar.org/paper/2b965ee9a367efadd047a858593af05dd737ee1a

How researchers see this paper

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In Biotechnology

1. H. Sharma
2. H. Daniell
3. J. Grevich
4. P. Christou
5. E. Stoger
6. J. Crouch
7. K. Sharma
8. P. Jauhar

In Molecular Biology

1. H. Sharma
2. H. Daniell
3. J. Grevich
4. P. Christou
5. E. Stoger
6. J. Crouch
7. Ralph Bock
8. G. Bonnett

In Plant Science

1. H. Sharma
2. H. Daniell
3. J. Grevich
4. J. Crouch
5. P. Christou
6. Ajay Kohli
7. K. Aitken
8. K. Sharma

Citations in the corpus

• Chloroplast genomes: diversity, evolution, and applications in genetic engineering (2016, background): “e–g Overexpression of the Bt cry2Aa2 operon in chlorop bioassays with the Helicoverpa zea, f eating the transplastomic leaf kills t caterpillar [137].”
• Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors (2004, background): “…et al. 1994)) were shown to express foreign proteins at extraordinarily high levels (Daniell et al. 1988; De Cosa et al. 2001; DeGray et al. 2001; Kota et al. 1999; McBride et al. 1995; Staub and Maliga 1993), reaching up to 46.1% of total soluble protein (tsp) in some cases (De Cosa et al. 2001).”
• Comprehending crystalline β-carotene accumulation by comparing engineered cell models and the natural carotenoid-rich system of citrus (2011, unknown): “The transcript abundance was normalized against peanut Ubiquitin (Luo et al. , 2005) transcript levels.”
• Particle bombardment and the genetic enhancement of crops: myths and realities (2005, background): “Transplastomic plants have been generated successfully to confer increased pest resistance (McBride et al. 1995; Kota et al. 1999; DeCosa et al. 2001), herbicide resistance (Daniell et al.”
• Genetic Transformation and Hybridization (2002, unknown): “It has been reported that up to 40% recombinant protein can accumulate in transplastomic chloroplasts (De Cosa et. al.”
• Sugarcane biotechnology: The challenges and opportunities (2005, background): “In another impressive example, transplastomic tobacco plants transformed with the Bt cry2Aa2 produced Bt toxin at 45.3% of the total soluble protein (De Cosa et al., 2001).”
• Chloroplast Genetic Engineering: Recent Advances and Future Perspectives (2005, background): “Foreign proteins have been shown to accumulate as high as 46 percent of the total soluble protein (tsp) (De Cosa et al., 2001).”
• Accumulation of trehalose within transgenic chloroplasts confers drought tolerance (2004, background): “Such variation in levels of foreign proteins within transgenic chloroplasts (100- fold) has been reported previously (Daniell et al. 2001a; De Cosa et al. 2001; Daniell et al. 2001d; Sidorov et al. 1999).”
• Protein crystallization in vivo (2005, background): “Interestingly, when expressed in a variety of other organisms (even plants) crystal formation can still occur [ 34 ].”
• Genetic Transformation of Crops for Insect Resistance: Potential and Limitations (2004, background): “3% of the total protein in mature leaves, which resulted in 100% mortality of cotton bollworm and beet armyworm ( Cosa et al. , 2002 ).”