Chloroplast expression of His-tagged GUS-fusions: a general strategy to overproduce and purify foreign proteins using transplastomic plants as bioreactors

By S. Leelavathi, V. S. Reddy. Molecular breeding, 2004

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Plant-based production of biopharmaceuticals. (2004, methodology): “Recent strategies that have been described include the expression of His-tagged GUS-fusion proteins in tobacco chloroplasts [58], the extraction of Histagged proteins by foam fractionation [59], and the release of recombinant proteins using a modified intein expression system [60 ].”
Chloroplast genomes: diversity, evolution, and applications in genetic engineering (2016, background): “rbcL/accD IFN-γ PpsbA/TpsbA 6 % TSP Protection of human lung carcinoma cells against infection by encephalomyocarditis virus [293]”
Chloroplast Genetic Engineering: Recent Advances and Future Perspectives (2005, background): “…Fernandez San-Millan et al., 2003), somatotropin (7%, Staub et al., 2000), interferon-alpha (19%, Daniell, Carmona-Sanchez, and Burns, 2004), interferon-gamma (6%, Leelavathi and Reddy, 2003), and antimicrobial peptide (21.5%, DeGray et al., 2001) facilitates efficient and economic purification.”
Genetic Transformation and Hybridization (2002, unknown): “Studies suggested that GUS fusion offers an attractive and effective system to increase the production of low expressing protein/polypeptide in transgenic plants (Leelavathi and Reddy 2003; Okamoto et al. 1998; Suo et al. 2006).”