Single-Cell Transcriptome Atlas of Murine Endothelial Cells.

By J. Kalucka, L. D. de Rooij, J. Goveia, Katerina Rohlenova, Sébastien J. Dumas, Elda Meta, Nadine V. Conchinha, Federico Taverna, L. Teuwen, K. Veys and 25 more. Cell, 2020

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The heterogeneity of endothelial cells (ECs) across tissues remains incompletely inventoried. We constructed an atlas of >32,000 single-EC transcriptomes from 11 mouse tissues and identified 78 EC subclusters, including Aqp7+ intestinal capillaries and angiogenic ECs in healthy tissues. ECs from brain/testis, liver/spleen, small intestine/colon, and skeletal muscle/heart pairwise expressed partially overlapping marker genes. Arterial, venous, and lymphatic ECs shared more markers in more tissues than did heterogeneous capillary ECs. ECs from different vascular beds (arteries, capillaries, veins, lymphatics) exhibited transcriptome similarity across tissues, but the tissue (rather than the vessel) type contributed to the EC heterogeneity. Metabolic transcriptome analysis revealed a similar tissue-grouping phenomenon of ECs and heterogeneous metabolic gene signatures in ECs between tissues and between vascular beds within a single tissue in a tissue-type-dependent pattern. The EC atlas taxonomy enabled identification of EC subclusters in public scRNA-seq datasets and provides a powerful discovery tool and resource value.

Subfields: Immunology, Molecular Biology

View Paper: https://www.semanticscholar.org/paper/2a2ffdf54cd6a75a85cababac50284b397fd72f1

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Citations in the corpus

Spatiotemporal analysis of human intestinal development at single-cell resolution (2021, background): “Similarly, in arterial clusters a spectrum of gene expression was seen ranging in expression of large arterial vessel genes (HEY1, GJA5) through to genes reported in capillary endothelial cells (VWA1, RBP7) (Buschmann et al., 2010; Hu et al., 2017; Kalucka et al., 2020).”
Endothelial Dysfunction in Atherosclerotic Cardiovascular Diseases and Beyond: From Mechanism to Pharmacotherapies (2021, background): “…et al., 2020). scRNA-seq has also revealed that endothelial cells are metabolically plastic and active in regulating multitissue homeostasis (Kalucka et al., 2020; Rohlenova et al., 2020). scRNA-seq is also successful in revealing endothelial cellular heterogeneity in a context-dependent…”